PMID- 21281598
OWN - NLM
STAT- MEDLINE
DCOM- 20110511
LR  - 20211203
IS  - 1096-0384 (Electronic)
IS  - 0003-9861 (Linking)
VI  - 508
IP  - 1
DP  - 2011 Apr 1
TI  - Secretion of three enzymes for fatty acid synthesis into mouse milk in 
      association with fat globules, and rapid decrease of the secreted enzymes by 
      treatment with rapamycin.
PG  - 87-92
LID - 10.1016/j.abb.2011.01.015 [doi]
AB  - The mammary epithelium produces numerous lipid droplets during lactation and 
      secretes them in plasma membrane-enclosed vesicles known as milk fat globules. 
      The biogenesis of such fat globules is considered to provide a model for 
      clarifying the mechanisms of lipogenesis in mammals. In the present study, we 
      identified acetyl coenzyme A carboxylase, ATP citrate lyase, and fatty acid 
      synthase in mouse milk. Fractionation of milk showed that these three enzymes 
      were located predominantly in milk fat globules. The three enzymes were resistant 
      to trypsin digestion without Triton X-100, indicating that they were not located 
      on the outer surface of the globules and thus associated with the precursors of 
      the globules before secretion. When a low dose of rapamycin, an inhibitor of the 
      mammalian target of rapamycin (mTOR), was injected into lactating mice, the 
      levels of the three enzymes in milk were decreased within 3h after injection. 
      Since the protein levels of the three enzymes in tissues were not obviously 
      altered by this short-term treatment, known transcriptional control by mTOR 
      signaling was unlikely to account for this decrease in their levels in milk. Our 
      findings suggest a new, putatively mTOR-dependent localization of the three 
      enzymes for de novo lipogenesis.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Moriya, Hitomi
AU  - Moriya H
AD  - Department of Applied Molecular Biosciences, Graduate School of Bioagricultural 
      Sciences, Nagoya University, Nagoya 464-8601, Japan.
FAU - Uchida, Kana
AU  - Uchida K
FAU - Okajima, Tetsuya
AU  - Okajima T
FAU - Matsuda, Tsukasa
AU  - Matsuda T
FAU - Nadano, Daita
AU  - Nadano D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110131
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Enzymes)
RN  - 0 (Fatty Acids)
RN  - 0 (Glycolipids)
RN  - 0 (Glycoproteins)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (milk fat globule)
RN  - EC 2.3.1.85 (Fatty Acid Synthases)
RN  - EC 2.3.3.8 (ATP Citrate (pro-S)-Lyase)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 6.4.1.2 (Acetyl-CoA Carboxylase)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - ATP Citrate (pro-S)-Lyase/metabolism
MH  - Acetyl-CoA Carboxylase/metabolism
MH  - Animals
MH  - Enzymes/*metabolism
MH  - Fatty Acid Synthases/metabolism
MH  - Fatty Acids/*biosynthesis
MH  - Female
MH  - Glycolipids/*metabolism
MH  - Glycoproteins/*metabolism
MH  - Lipid Droplets
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Milk/*enzymology
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Sirolimus/*pharmacology
MH  - TOR Serine-Threonine Kinases/antagonists & inhibitors
MH  - Time Factors
EDAT- 2011/02/02 06:00
MHDA- 2011/05/12 06:00
CRDT- 2011/02/02 06:00
PHST- 2010/10/26 00:00 [received]
PHST- 2011/01/21 00:00 [revised]
PHST- 2011/01/24 00:00 [accepted]
PHST- 2011/02/02 06:00 [entrez]
PHST- 2011/02/02 06:00 [pubmed]
PHST- 2011/05/12 06:00 [medline]
AID - S0003-9861(11)00039-7 [pii]
AID - 10.1016/j.abb.2011.01.015 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2011 Apr 1;508(1):87-92. doi: 10.1016/j.abb.2011.01.015. 
      Epub 2011 Jan 31.