PMID- 21282982
OWN - NLM
STAT- MEDLINE
DCOM- 20110912
LR  - 20211020
IS  - 1555-8584 (Electronic)
IS  - 1547-6286 (Print)
IS  - 1547-6286 (Linking)
VI  - 8
IP  - 1
DP  - 2011 Jan-Feb
TI  - snRNA-specific role of SMN in trypanosome snRNP biogenesis in vivo.
PG  - 90-100
AB  - Pre-mRNA splicing in trypanosomes requires the SMN-mediated assembly of small 
      nuclear ribonucleoproteins (snRNPs). In contrast to higher eukaryotes, the 
      cellular localization of snRNP biogenesis and the involvement of 
      nuclear-cytoplasmic trafficking in trypanosomes are controversial. By using RNAi 
      knockdown of SMN in T. brucei to investigate its functional role in snRNP 
      assembly, we found dramatic changes in the steady-state levels of snRNAs and 
      snRNPs: The SL RNA accumulates, whereas U1, U4, and U5 snRNA levels decrease, and 
      Sm core assembly in particular of the SL RNA is strongly reduced. In addition, 
      SMN depletion blocks U4/U6 di-snRNP formation; the variant Sm core of the U2 
      snRNP, however, still forms efficiently after SMN knockdown. Concerning the 
      longstanding question, whether nuclear-cytoplasmic trafficking is involved in 
      trypanosomal snRNP biogenesis, fluorescence in situ hybridization (FISH) and 
      immunofluorescence assays revealed that the SL RNA genes and transcripts 
      colocalize with SMN. Remarkably, SMN silencing leads to a nucleoplasmic 
      accumulation of both SL RNA and the Sm proteins. In sum, our data demonstrate an 
      essential and snRNA-selective role of SMN in snRNP biogenesis in vivo and 
      strongly argue for a nucleoplasmic Sm core assembly of the SL RNP.
FAU - Jae, Nicolas
AU  - Jae N
AD  - Institut fur Biochemie, Justus-Liebig-Universitat Giessen, Giessen, Germany.
FAU - Preusser, Christian
AU  - Preusser C
FAU - Kruger, Timothy
AU  - Kruger T
FAU - Tkacz, Itai Dov
AU  - Tkacz ID
FAU - Engstler, Markus
AU  - Engstler M
FAU - Michaeli, Shulamit
AU  - Michaeli S
FAU - Bindereif, Albrecht
AU  - Bindereif A
LA  - eng
GR  - Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110101
PL  - United States
TA  - RNA Biol
JT  - RNA biology
JID - 101235328
RN  - 0 (RNA, Protozoan)
RN  - 0 (RNA, Small Nuclear)
RN  - 0 (RNA, Spliced Leader)
RN  - 0 (Ribonucleoproteins, Small Nuclear)
RN  - 0 (SMN Complex Proteins)
SB  - IM
MH  - Cell Nucleus/genetics/metabolism
MH  - Cells, Cultured
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation
MH  - Gene Knockdown Techniques
MH  - Genetic Loci
MH  - In Situ Hybridization, Fluorescence
MH  - RNA Interference
MH  - RNA Splicing
MH  - RNA, Protozoan/genetics/*metabolism
MH  - RNA, Small Nuclear/analysis/*metabolism
MH  - RNA, Spliced Leader/metabolism
MH  - Ribonucleoproteins, Small Nuclear/*metabolism
MH  - SMN Complex Proteins/genetics/*metabolism
MH  - Trypanosoma brucei brucei/*genetics/metabolism
PMC - PMC3127081
EDAT- 2011/02/02 06:00
MHDA- 2011/09/13 06:00
PMCR- 2011/01/01
CRDT- 2011/02/02 06:00
PHST- 2011/02/02 06:00 [entrez]
PHST- 2011/02/02 06:00 [pubmed]
PHST- 2011/09/13 06:00 [medline]
PHST- 2011/01/01 00:00 [pmc-release]
AID - 13985 [pii]
AID - 1547-6286-8-1-18 [pii]
AID - 10.4161/rna.8.1.13985 [doi]
PST - ppublish
SO  - RNA Biol. 2011 Jan-Feb;8(1):90-100. doi: 10.4161/rna.8.1.13985. Epub 2011 Jan 1.