PMID- 21287518
OWN - NLM
STAT- MEDLINE
DCOM- 20110414
LR  - 20110202
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 28
IP  - 1
DP  - 2011 Feb
TI  - [Sequence analysis of a novel human leukocyte antigen allele B*5827].
PG  - 88-91
LID - 10.3760/cma.j.issn.1003-9406.2011.01.020 [doi]
AB  - OBJECTIVE: To investigate the molecular basis for a novel human leukocyte antigen 
      (HLA) allele B*5827. METHODS: DNA from the proband was analyzed by polymerase 
      chain reaction-sequence specific oligonucleotide (PCR-SSO) typing. The amplified 
      product was sequenced bidirectionally. RESULTS: Abnormal HLA-B locus was observed 
      and its nucleotide sequence was different from the known HLA-B allele sequences, 
      with highest homology to HLA-B*5820 allele. It differs from HLA-B*5820 by 8 
      nucleotide substitutions in exon 3, i.e., nt 290 (G > C), nt 346 (T > A), nt 390 
      (A > C), nt 404 (G > C), nt 413 (C > G), nt 471 (A > G), nt 486 (A > G) and nt 
      487 (C > A), resulting in an amino acid change from ser > arg at nt 97, phe >tyr 
      at nt 115, ser > arg at nt 130, thr > ala at nt 157 and thr > glu at nt 162. 
      Nucleotide differences of nt 404 (G > C) and nt 413( C > G) did not change amino 
      acid. CONCLUSION: The sequences of the novel allele have been submitted to 
      GenBank (access No.GU071234). A novel HLA class I allele B*5827 has been 
      officially assigned by the WHO HLA Nomenclature Committee in Jan. 2010.
FAU - Lu, Chao-xia
AU  - Lu CX
AD  - Shandong Umbilical Cord Blood Bank, Jinan, Shandong, 250102 PR China.
FAU - Zhu, Na
AU  - Zhu N
FAU - Zhang, Qian
AU  - Zhang Q
FAU - Huang, Hong
AU  - Huang H
FAU - Ke, Bing-shen
AU  - Ke BS
FAU - Hou, Huai-shui
AU  - Hou HS
FAU - Shen, Bai-jun
AU  - Shen BJ
LA  - chi
SI  - GENBANK/GU071234
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (HLA-B Antigens)
SB  - IM
MH  - *Alleles
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Genotype
MH  - HLA-B Antigens/chemistry/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
EDAT- 2011/02/03 06:00
MHDA- 2011/04/16 06:00
CRDT- 2011/02/03 06:00
PHST- 2011/02/03 06:00 [entrez]
PHST- 2011/02/03 06:00 [pubmed]
PHST- 2011/04/16 06:00 [medline]
AID - 940628020 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2011.01.020 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2011 Feb;28(1):88-91. doi: 
      10.3760/cma.j.issn.1003-9406.2011.01.020.