PMID- 21288071
OWN - NLM
STAT- MEDLINE
DCOM- 20120426
LR  - 20211020
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 21
IP  - 1
DP  - 2012 Jan
TI  - Analyses of donor-derived keratinocytes in hairy and nonhairy skin biopsies of 
      female patients following allogeneic male bone marrow transplantation.
PG  - 152-7
LID - 10.1089/scd.2010.0593 [doi]
AB  - Skin samples taken from 6 female patients receiving allogeneic bone marrow 
      transplants (BMT) from male siblings (n=5) or from unrelated human leukocyte 
      antigen (HLA)-matched male donor (n=1) due to hematological malignancies were 
      studied for the presence of donor cells. One nontransplanted male and 1 female 
      control that received female BM were used as further controls of the technique. 
      Skin biopsies were taken from the scalp and the back from each patient 12-16 
      years after the successful BMT. We have found donor chimerism in all of the 6 
      patients in both of their biopsies. Using single and double immunostainings in 
      combination with Y chromosome hybridization, we observed that there are 
      cytokeratin-expressing donor-derived cells in the epidermis of all the 6 
      patients, the numbers being slightly higher in the scalp (0.37%-1.78%) than in 
      the back (0.32%-1.08%) biopsies. The indication for BMT, and the age of the 
      patient did not seem to have any effect on the numbers found. A few of the 
      double-labeled cells also stained for Ki67, a marker of cellular proliferation, 
      suggesting that the engrafted cells were able to further divide in the epidermis. 
      In 2 patients we observed patches of donor keratinocytes within the epidermis, 
      suggesting a clonal origin. We conclude that in agreement with some and in 
      contrast to other published studies, BM-derived circulating cells are able to 
      engraft in the human skin and to further proliferate there and thus contribute to 
      tissue renewal. These data raise the possibility to use BM cells in regenerative 
      medicine to help in extended injuries, large surface burns, or lack of skin due 
      to other reasons.
FAU - Nemeth, Krisztian
AU  - Nemeth K
AD  - Craniofacial and Skeletal Diseases Branch, National Institutes of Dental and 
      Craniofacial Research, Bethesda, Maryland 20892, USA.
FAU - Key, Sharon
AU  - Key S
FAU - Bottlik, Gyula
AU  - Bottlik G
FAU - Masszi, Tamas
AU  - Masszi T
FAU - Mezey, Eva
AU  - Mezey E
FAU - Karpati, Sarolta
AU  - Karpati S
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20110317
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Adult
MH  - Back/pathology
MH  - Biopsy
MH  - *Bone Marrow Transplantation
MH  - Chimera
MH  - Chromosomes, Human, Y/metabolism
MH  - Female
MH  - Hematologic Neoplasms/therapy
MH  - Humans
MH  - Keratinocytes/*metabolism
MH  - Keratins/metabolism
MH  - Male
MH  - Middle Aged
MH  - Scalp/pathology
MH  - Skin/*pathology
MH  - Tissue Donors
MH  - Transplantation, Homologous
PMC - PMC3245666
EDAT- 2011/02/04 06:00
MHDA- 2012/04/27 06:00
PMCR- 2013/01/01
CRDT- 2011/02/04 06:00
PHST- 2011/02/04 06:00 [entrez]
PHST- 2011/02/04 06:00 [pubmed]
PHST- 2012/04/27 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 10.1089/scd.2010.0593 [pii]
AID - 10.1089/scd.2010.0593 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2012 Jan;21(1):152-7. doi: 10.1089/scd.2010.0593. Epub 2011 Mar 
      17.