PMID- 21289263
OWN - NLM
STAT- MEDLINE
DCOM- 20110614
LR  - 20140730
IS  - 1945-7197 (Electronic)
IS  - 0021-972X (Linking)
VI  - 96
IP  - 4
DP  - 2011 Apr
TI  - The effect of very-low-calorie diet on mRNA expression of inflammation-related 
      genes in subcutaneous adipose tissue and peripheral monocytes of obese patients 
      with type 2 diabetes mellitus.
PG  - E606-13
LID - 10.1210/jc.2010-1858 [doi]
AB  - CONTEXT: Low-grade inflammation links obesity, type 2 diabetes mellitus (T2DM), 
      and cardiovascular diseases. OBJECTIVE: To explore the expression profile of 
      genes involved in inflammatory pathways in adipose tissue and peripheral 
      monocytes (PM) of obese patients with and without T2DM at baseline and after 
      dietary intervention. DESIGN: Two-week intervention study with very-low-calorie 
      diet (VLCD). SETTING: University hospital. PATIENTS: Twelve obese females with 
      T2DM, 8 obese nondiabetic females (OB) and 15 healthy age-matched females. 
      INTERVENTION: Two weeks of VLCD (2500 kJ/d). MAIN OUTCOME MEASURES: Metabolic 
      parameters, circulating cytokines, hormones, and mRNA expression of 39 genes in 
      sc adipose tissue (SCAT) and PM. RESULTS: Both T2DM and OB group had 
      significantly increased serum concentrations of circulating proinflammatory 
      factors (C-reactive protein, TNFalpha, IL-6, IL-8), mRNA expression of macrophage 
      antigen CD68 and proinflammatory chemokines (CCL-2, -3, -7, -8, -17, -22) in SCAT 
      and complementary chemokine receptors (CCR-1, -2, -3, -5) and other 
      proinflammatory receptors (toll-like receptor 2 and 4, TNF receptor superfamily 
      1A and 1B, IL-6R) in PM, with OB group showing less pronounced chemoattracting 
      and proinflammatory profile compared to T2DM group. In T2DM patients VLCD 
      decreased body weight, improved metabolic profile, and decreased mRNA expression 
      of up-regulated CCRs in PM and chemokines [CCL 8, chemokine (C-X-C motif) ligand 
      10] in SCAT. VLCD markedly increased mRNA expression of T-lymphocyte attracting 
      chemokine CCL-17 in SCAT. CONCLUSION: Obese patients with and without T2DM have 
      increased mRNA expression of chemotactic and proinflammatory factors in SCAT and 
      expression of corresponding receptors in PM. Two weeks of VLCD significantly 
      improved this profile in T2DM patients.
FAU - Mraz, M
AU  - Mraz M
AD  - Third Department of Medicine, General University Hospital and First Medical 
      Faculty, Charles University, 128 08 Prague 2, Czech Republic.
FAU - Lacinova, Z
AU  - Lacinova Z
FAU - Drapalova, J
AU  - Drapalova J
FAU - Haluzikova, D
AU  - Haluzikova D
FAU - Horinek, A
AU  - Horinek A
FAU - Matoulek, M
AU  - Matoulek M
FAU - Trachta, P
AU  - Trachta P
FAU - Kavalkova, P
AU  - Kavalkova P
FAU - Svacina, S
AU  - Svacina S
FAU - Haluzik, M
AU  - Haluzik M
LA  - eng
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110202
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Adipokines)
RN  - 0 (Chemokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Adipokine)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Adipokines/genetics/metabolism
MH  - *Caloric Restriction
MH  - Chemokines/genetics/metabolism
MH  - Diabetes Mellitus, Type 2/blood/complications/*diet therapy/genetics
MH  - Diet, Reducing
MH  - Female
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Inflammation/complications/*genetics/metabolism
MH  - Inflammation Mediators/metabolism
MH  - Middle Aged
MH  - Monocytes/*metabolism
MH  - Obesity/blood/complications/*diet therapy/genetics
MH  - RNA, Messenger/*genetics/metabolism
MH  - Receptors, Adipokine/genetics/metabolism
MH  - Receptors, Chemokine/genetics/metabolism
MH  - Subcutaneous Fat/*metabolism
EDAT- 2011/02/04 06:00
MHDA- 2011/06/15 06:00
CRDT- 2011/02/04 06:00
PHST- 2011/02/04 06:00 [entrez]
PHST- 2011/02/04 06:00 [pubmed]
PHST- 2011/06/15 06:00 [medline]
AID - jc.2010-1858 [pii]
AID - 10.1210/jc.2010-1858 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2011 Apr;96(4):E606-13. doi: 10.1210/jc.2010-1858. Epub 
      2011 Feb 2.