PMID- 21291098
OWN - NLM
STAT- MEDLINE
DCOM- 20110301
LR  - 20171116
IS  - 0043-3144 (Print)
IS  - 0043-3144 (Linking)
VI  - 59
IP  - 3
DP  - 2010 Jun
TI  - Investigation of CTLA-4 and CD28 gene polymorphisms in patients with diabetes 
      mellitus type 2 using PCR-RFLP in a Turkish population.
PG  - 235-40
AB  - OBJECTIVE: The aim of this study is to investigate whether specific polymorphisms 
      in the CTLA-4 and CD28 gene are associated with Type 2 diabetes mellitus (T2DM). 
      METHODS: Blood samples were collected from 241 individuals (72 patients with T2DM 
      and 169 healthy individuals) and DNA was isolated. A polymerase chain 
      reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to 
      detect the frequencies of CTLA-4 NM_005214.3:c.49A > G and c.-319C > T, and CD28 
      NM_006139.1:c.534+17T > C polymorphisms in T2DM patients in the Sanliurfa 
      Population. RESULTS: The data suggested that body mass index (BMI), total 
      cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c) 
      and haemoglobin A1c (HbA1c) were significantly higher in T2DM patients than in 
      the control individuals (p < 0.05). No significant differences were observed for 
      the frequencies of c.49A > G, c.-319C > T genotype and allele of CTLA-4 gene and 
      c.534+17T > C of the CD28 gene in T2DM patients compared to healthy individuals 
      (p > 0.05). CONCLUSION: The CTLA-4 gene c.49A > G and c.-319C > T and CD28 gene 
      c.534+ 17T > C polymorphisms did not represent an important risk factor for this 
      disease in a group of the Turkish population.
FAU - Uzer, E
AU  - Uzer E
AD  - Department of Internal Medicine, Faculty of Medicine, Harran University, 
      Sanliurfa, Turkey.
FAU - Dilmec, F
AU  - Dilmec F
FAU - Akkafa, F
AU  - Akkafa F
FAU - Boduroglu, O
AU  - Boduroglu O
FAU - van Kuilenburg, A B P
AU  - van Kuilenburg AB
LA  - eng
PT  - Journal Article
PL  - Jamaica
TA  - West Indian Med J
JT  - The West Indian medical journal
JID - 0417410
RN  - 0 (Antigens, CD)
RN  - 0 (CD28 Antigens)
RN  - 0 (CTLA-4 Antigen)
RN  - 0 (CTLA4 protein, human)
SB  - IM
MH  - Aged
MH  - Antigens, CD/*genetics
MH  - CD28 Antigens/*genetics
MH  - CTLA-4 Antigen
MH  - Diabetes Mellitus, Type 2/*genetics
MH  - Female
MH  - Gene Frequency
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Genetic/genetics
MH  - Polymorphism, Restriction Fragment Length
MH  - *Polymorphism, Single Nucleotide
MH  - Turkey
EDAT- 2011/02/05 06:00
MHDA- 2011/03/02 06:00
CRDT- 2011/02/05 06:00
PHST- 2011/02/05 06:00 [entrez]
PHST- 2011/02/05 06:00 [pubmed]
PHST- 2011/03/02 06:00 [medline]
AID - 1232 [pii]
PST - ppublish
SO  - West Indian Med J. 2010 Jun;59(3):235-40.