PMID- 21291569
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 4
IP  - 1
DP  - 2011 Feb 3
TI  - Evaluation of chronic lymphocytic leukemia by BAC-based microarray analysis.
PG  - 4
LID - 10.1186/1755-8166-4-4 [doi]
AB  - BACKGROUND: Chronic lymphocytic leukemia (CLL) is a highly variable disease with 
      life expectancies ranging from months to decades. Cytogenetic findings play an 
      integral role in defining the prognostic significance and treatment for 
      individual patients. RESULTS: We have evaluated 25 clinical cases from a tertiary 
      cancer center that have an established diagnosis of CLL and for which there was 
      prior cytogenetic and/or fluorescence in situ hybridization (FISH) data. We 
      performed microarray-based comparative genomic hybridization (aCGH) using a 
      bacterial artificial chromosome (BAC)-based microarray designed for the detection 
      of known constitutional genetic syndromes. In 15 of the 25 cases, aCGH detected 
      all copy number imbalances identified by prior cytogenetic and/or FISH studies. 
      For the majority of those not detected, the aberrations were present at low 
      levels of mosaicism. Furthermore, for 15 of the 25 cases, additional 
      abnormalities were detected. Four of those cases had deletions that mapped to 
      intervals implicated in inherited predisposition to CLL. For most cases, aCGH was 
      able to detect abnormalities present in as few as 10% of cells. Although changes 
      in ploidy are not easily discernable by aCGH, results for two cases illustrate 
      the detection of additional copy gains and losses present within a mosaic 
      tetraploid cell population. CONCLUSIONS: Our results illustrate the successful 
      evaluation of CLL using a microarray optimized for the interrogation of inherited 
      disorders and the identification of alterations with possible relevance to CLL 
      susceptibility.
FAU - Schultz, Roger A
AU  - Schultz RA
AD  - Signature Genomics, 2820 N, Astor St,, Spokane, WA, 99207, USA. 
      lisa.shaffer@perkinelmer.com.
FAU - Delioukina, Maria
AU  - Delioukina M
FAU - Gaal, Karl
AU  - Gaal K
FAU - Bedell, Victoria
AU  - Bedell V
FAU - Smith, David D
AU  - Smith DD
FAU - Forman, Stephen J
AU  - Forman SJ
FAU - McDaniel, Lisa D
AU  - McDaniel LD
FAU - Ballif, Blake C
AU  - Ballif BC
FAU - Shaffer, Lisa G
AU  - Shaffer LG
FAU - Slovak, Marilyn L
AU  - Slovak ML
LA  - eng
PT  - Journal Article
DEP - 20110203
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC3045370
EDAT- 2011/02/05 06:00
MHDA- 2011/02/05 06:01
PMCR- 2011/02/03
CRDT- 2011/02/05 06:00
PHST- 2010/11/12 00:00 [received]
PHST- 2011/02/03 00:00 [accepted]
PHST- 2011/02/05 06:00 [entrez]
PHST- 2011/02/05 06:00 [pubmed]
PHST- 2011/02/05 06:01 [medline]
PHST- 2011/02/03 00:00 [pmc-release]
AID - 1755-8166-4-4 [pii]
AID - 10.1186/1755-8166-4-4 [doi]
PST - epublish
SO  - Mol Cytogenet. 2011 Feb 3;4(1):4. doi: 10.1186/1755-8166-4-4.