PMID- 21292037
OWN - NLM
STAT- MEDLINE
DCOM- 20110725
LR  - 20110412
IS  - 1872-8278 (Electronic)
IS  - 1567-7249 (Linking)
VI  - 11
IP  - 3
DP  - 2011 May
TI  - A genome-wide shRNA screen for new OxPhos related genes.
PG  - 467-75
LID - 10.1016/j.mito.2011.01.007 [doi]
AB  - The mitochondrial oxidative phosphorylation (OxPhos) system produces most of the 
      ATP required by the cell. The structural proteins of the OxPhos holoenzymes are 
      well known, but important aspects of their biogenesis and regulation remain to be 
      uncovered and a significant fraction of mitochondrial proteins have yet to be 
      identified. We have used a high throughput, genome-wide RNA interference (RNAi) 
      approach to identify new OxPhos-related genes. We transduced a mouse fibroblast 
      cell line with a lentiviral-based shRNA-library, and screened the cell population 
      for growth impairment in galactose-based medium, which requires an intact OxPhos 
      system. Candidate genes were ranked according to their co-expression with known 
      genes encoding OxPhos mitochondria-located proteins. For the top ranking 
      candidates the cellular process in which they are involved was evaluated. Our 
      results show that the use of genome-wide RNAi together with screening for 
      deficient growth in galactose medium is a suitable approach to identifying 
      OxPhos-related and cellular energy metabolism-related genes. Interestingly also 
      ubiquitin-proteasome related genes were selected.
CI  - Copyright (c) 2011 (c) Elsevier B.V. and Mitochondria Research Society. All rights 
      reserved. Published by Elsevier B.V. All rights reserved.
FAU - Bayona-Bafaluy, Maria Pilar
AU  - Bayona-Bafaluy MP
AD  - Departamento de Bioquimica y Biologia Molecular y Celular. Facultad de Ciencias, 
      Universidad de Zaragoza, Zaragoza 50013, Spain.
FAU - Sanchez-Cabo, Fatima
AU  - Sanchez-Cabo F
FAU - Fernandez-Silva, Patricio
AU  - Fernandez-Silva P
FAU - Perez-Martos, Acisclo
AU  - Perez-Martos A
FAU - Enriquez, Jose Antonio
AU  - Enriquez JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110201
PL  - Netherlands
TA  - Mitochondrion
JT  - Mitochondrion
JID - 100968751
RN  - 0 (Electron Transport Chain Complex Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - EC 1.- (Oxidoreductases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Electron Transport Chain Complex Proteins/genetics/*metabolism
MH  - Fibroblasts/metabolism
MH  - Genetic Vectors
MH  - High-Throughput Screening Assays/*methods
MH  - Lentivirus/genetics
MH  - Mice
MH  - Mitochondria/*enzymology/metabolism
MH  - *Oxidative Phosphorylation
MH  - Oxidoreductases/*antagonists & inhibitors/genetics
MH  - *RNA Interference
MH  - RNA, Small Interfering/genetics/*metabolism
MH  - Transduction, Genetic
EDAT- 2011/02/05 06:00
MHDA- 2011/07/26 06:00
CRDT- 2011/02/05 06:00
PHST- 2010/07/21 00:00 [received]
PHST- 2010/11/24 00:00 [revised]
PHST- 2011/01/24 00:00 [accepted]
PHST- 2011/02/05 06:00 [entrez]
PHST- 2011/02/05 06:00 [pubmed]
PHST- 2011/07/26 06:00 [medline]
AID - S1567-7249(11)00028-6 [pii]
AID - 10.1016/j.mito.2011.01.007 [doi]
PST - ppublish
SO  - Mitochondrion. 2011 May;11(3):467-75. doi: 10.1016/j.mito.2011.01.007. Epub 2011 
      Feb 1.