PMID- 2129226
OWN - NLM
STAT- MEDLINE
DCOM- 19910821
LR  - 20220215
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 110
IP  - 4
DP  - 1990 Dec
TI  - Establishment of germ-line-competent embryonic stem (ES) cells using 
      differentiation inhibiting activity.
PG  - 1341-8
AB  - The regulatory factor Differentiation Inhibiting Activity/Leukaemia Inhibitory 
      Factor (DIA/LIF) suppresses the differentiation of cultured embryonic stem (ES) 
      cells. In the present study, it is shown that ES cell lines can be derived and 
      maintained in the absence of feeder layers using medium supplemented with 
      purified DIA/LIF. These cells can differentiate normally in vitro and in vivo and 
      they retain the capacity for germ-line transmission. DIA/LIF therefore fulfils 
      the essential function of feeders in the isolation of pluripotential stem cells.
FAU - Nichols, J
AU  - Nichols J
AD  - Department of Zoology, University of Oxford, UK.
FAU - Evans, E P
AU  - Evans EP
FAU - Smith, A G
AU  - Smith AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Culture Media)
RN  - 0 (Growth Inhibitors)
RN  - 0 (Interleukin-6)
RN  - 0 (Leukemia Inhibitory Factor)
RN  - 0 (Lif protein, mouse)
RN  - 0 (Lymphokines)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chimera
MH  - Culture Media
MH  - *Growth Inhibitors
MH  - *Interleukin-6
MH  - Karyotyping
MH  - Leukemia Inhibitory Factor
MH  - Lymphokines/*physiology
MH  - Mice
MH  - Stem Cells/*physiology/ultrastructure
EDAT- 1990/12/01 00:00
MHDA- 1990/12/01 00:01
CRDT- 1990/12/01 00:00
PHST- 1990/12/01 00:00 [pubmed]
PHST- 1990/12/01 00:01 [medline]
PHST- 1990/12/01 00:00 [entrez]
AID - 10.1242/dev.110.4.1341 [doi]
PST - ppublish
SO  - Development. 1990 Dec;110(4):1341-8. doi: 10.1242/dev.110.4.1341.