PMID- 21293824
OWN - NLM
STAT- MEDLINE
DCOM- 20110610
LR  - 20161125
IS  - 1473-0189 (Electronic)
IS  - 1473-0189 (Linking)
VI  - 11
IP  - 6
DP  - 2011 Mar 21
TI  - Assay for molecular transport across gap junction channels in one-dimensional 
      cell arrays.
PG  - 1096-101
LID - 10.1039/c0lc00350f [doi]
AB  - Transport across gap junction channels (GJCs) between neighboring cells is 
      critical to synchronizing cell's electrical and metabolic activities and 
      maintaining cell homeostasis. Here we present a non-invasive microfluidic method 
      to measure molecular diffusion across GJCs in multiple 1D cell arrays in real 
      time. Using the chip, selective loading of a membrane permeant fluorescence dye 
      (carboxyfluorescein) in Normal Rat Kidney (NRK) cells shows that the dye was able 
      to diffuse through three cells along single cell chains in  approximately 35 minutes. 
      Application of 100 microM 2-aminoethoxydiphenyl borate (2-APB) reversibly inhibits 
      connexin-43 gap junctions in NRK cells; 0.8 mM 1-heptanol inhibits the diffusion 
      partially. The method offers rapid exchange of reagents, enabling sequential 
      screening of multiple gap junction specific drugs with only one preparation of 
      cells. It is capable of measuring gap junction mediated diffusion between single 
      cells.
CI  - This journal is (c) The Royal Society of Chemistry 2011
FAU - Ye, Nannan
AU  - Ye N
AD  - Department of Physiology and Biophysics, SUNY-Buffalo, Buffalo, NY 14260, USA.
FAU - Bathany, Cedric
AU  - Bathany C
FAU - Hua, Susan Z
AU  - Hua SZ
LA  - eng
GR  - R21 NS062657/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110204
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (Boron Compounds)
RN  - 0 (Connexin 43)
RN  - 0 (Fluorescent Dyes)
RN  - 8JQ5607IO5 (Heptanol)
RN  - E4ES684O93 (2-aminoethoxydiphenyl borate)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Boron Compounds/chemistry/pharmacology
MH  - Cells, Cultured
MH  - Connexin 43/antagonists & inhibitors/metabolism
MH  - Diffusion
MH  - Fluorescent Dyes/chemistry
MH  - Gap Junctions/*physiology
MH  - Heptanol/chemistry
MH  - Rats
MH  - Tissue Array Analysis/instrumentation/*methods
EDAT- 2011/02/05 06:00
MHDA- 2011/06/11 06:00
CRDT- 2011/02/05 06:00
PHST- 2011/02/05 06:00 [entrez]
PHST- 2011/02/05 06:00 [pubmed]
PHST- 2011/06/11 06:00 [medline]
AID - 10.1039/c0lc00350f [doi]
PST - ppublish
SO  - Lab Chip. 2011 Mar 21;11(6):1096-101. doi: 10.1039/c0lc00350f. Epub 2011 Feb 4.