PMID- 21295817
OWN - NLM
STAT- MEDLINE
DCOM- 20110421
LR  - 20171116
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 83
IP  - 2
DP  - 2011 Mar
TI  - The microbiota of an unpolluted calcareous soil faces up chlorophenols: Evidences 
      of resistant strains with potential for bioremediation.
PG  - 104-16
LID - 10.1016/j.chemosphere.2011.01.016 [doi]
AB  - To highlight the effects of a variety of chlorophenols (CP) in relation to the 
      response of an indigenous bacterial community, an agricultural Mediterranean 
      calcareous soil has been studied in microcosms incubated under controlled 
      laboratory conditions. Soil samples were artificially polluted with 
      2-monochlorophenol (MCP), 2,4,6-trichlorophenol (TCP) and pentachlorophenol 
      (PCP), at concentrations ranging from 0.1 up to 5000 mg kg(-1). Both activity and 
      composition of the microbial community were assessed during several weeks, 
      respectively, by respirometric methods and PCR-DGGE analysis of extracted DNA and 
      RNA. Significant decreases in soil respirometric values and changes in the 
      bacterial community composition were observed at concentrations above 1000 mg 
      kg(-1) MCP and TCP, and above 100 mg kg(-1) PCP. However, the persistence of 
      several active bacterial populations in soil microcosms contaminated with high 
      concentration of CP, as indicated by DGGE fingerprints, suggested the capacity of 
      these native bacteria to survive in the presence of the pollutants, even without 
      a previous adaptation or contact with them. The isolation of potential CP 
      degraders was attempted by culture plating from microcosms incubated with high CP 
      concentrations. Twenty-three different isolates were screened for their 
      resistance to TCP and PCP. The most resistant isolates were identified as Kocuria 
      palustris, Lysobacter gummosus, Bacillus sp. and Pseudomonas putida, according to 
      16S rRNA gene homology. In addition, these four isolates also showed the capacity 
      to reduce the concentration of TCP and PCP from 15% to 30% after 5d of incubation 
      in laboratory assays (initial pollutant concentration of 50 mg L(-1)). Isolate 
      ITP29, which could be a novel species of Bacillus, has been revealed as the first 
      known member in this bacterial group with potential for CP bioremediation 
      applications, usually wide-spread in the soil natural communities, which has not 
      been reported to date as a CP degrader.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Caliz, Joan
AU  - Caliz J
AD  - Group of Molecular Microbial Ecology, Institute of Aquatic Ecology, University of 
      Girona, Avda Montilivi s/n, Girona, Spain.
FAU - Vila, Xavier
AU  - Vila X
FAU - Marti, Esther
AU  - Marti E
FAU - Sierra, Jordi
AU  - Sierra J
FAU - Nordgren, Johan
AU  - Nordgren J
FAU - Lindgren, Per-Eric
AU  - Lindgren PE
FAU - Baneras, Lluis
AU  - Baneras L
FAU - Montserrat, Genoveva
AU  - Montserrat G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110204
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Chlorophenols)
RN  - 0 (Soil)
RN  - 0 (Soil Pollutants)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Bacteria/classification/*genetics/isolation & purification
MH  - Bacterial Physiological Phenomena
MH  - Biodegradation, Environmental
MH  - Calcium Carbonate/analysis
MH  - Chlorophenols/analysis/*toxicity
MH  - Drug Tolerance/*genetics
MH  - Phylogeny
MH  - Soil/*chemistry
MH  - *Soil Microbiology
MH  - Soil Pollutants/analysis/*toxicity
EDAT- 2011/02/08 06:00
MHDA- 2011/04/22 06:00
CRDT- 2011/02/08 06:00
PHST- 2010/10/19 00:00 [received]
PHST- 2010/12/21 00:00 [revised]
PHST- 2011/01/05 00:00 [accepted]
PHST- 2011/02/08 06:00 [entrez]
PHST- 2011/02/08 06:00 [pubmed]
PHST- 2011/04/22 06:00 [medline]
AID - S0045-6535(11)00018-X [pii]
AID - 10.1016/j.chemosphere.2011.01.016 [doi]
PST - ppublish
SO  - Chemosphere. 2011 Mar;83(2):104-16. doi: 10.1016/j.chemosphere.2011.01.016. Epub 
      2011 Feb 4.