PMID- 21296388
OWN - NLM
STAT- MEDLINE
DCOM- 20110704
LR  - 20220331
IS  - 1527-9995 (Electronic)
IS  - 0090-4295 (Linking)
VI  - 77
IP  - 4
DP  - 2011 Apr
TI  - Clinical utility of fluorescence in situ hybridization for prediction of residual 
      tumor after transurethral resection of bladder urothelial carcinoma.
PG  - 855-9
LID - 10.1016/j.urology.2010.09.061 [doi]
AB  - OBJECTIVES: To evaluate the use of a multiprobe fluorescence in situ 
      hybridization (FISH) assay for predicting the residual tumor load after 
      transurethral resection (TUR) of bladder urothelial carcinoma (UC). METHODS: 
      Voided urine specimens were collected from 125 consecutive patients with 
      suspected UC who had been admitted for TUR. Of the 125 patients, 89 with UC 
      diagnosed underwent a second procedure (repeated TUR or cystectomy) 4-6 weeks 
      after the initial TUR and were included in the present study. Using the 
      pathologic findings from the second procedure, the patients were divided into 
      those with (n = 38) and those without (n = 51) residual tumor. Urine samples were 
      taken both before and after the initial TUR. The multiprobe FISH assay was 
      performed to detect aneuploidy of chromosomes 3, 7, and 17 and the loss of the 
      9p21 locus. RESULTS: Before the first TUR, no significant difference was found in 
      the FISH-positive percentage between those with and without residual tumor. After 
      the first TUR, the FISH-positive percentage in those with residual tumor was 
      significantly greater than in those without residual tumor (42.2% vs 17.6%, P = 
      .003). Moreover, before and after the initial TUR, the percentage of conversion 
      from FISH positive to FISH negative in those with residual tumor was 
      significantly lower than in those without residual tumor (28.9% vs 58.9%, P < 
      .001). No patients were observed with a FISH result of conversion from negative 
      to positive in those with and without residual tumor after the initial TUR. 
      CONCLUSIONS: FISH appears to be useful for the prediction of the presence of the 
      residual tumor load after TUR of bladder UC.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Ding, Tao
AU  - Ding T
AD  - Department of Urology, Second Xiangya Hospital, Central South University, 
      Changsha, China.
FAU - Wang, Yin-Kui
AU  - Wang YK
FAU - Cao, You-Han
AU  - Cao YH
FAU - Yang, Luo-Yan
AU  - Yang LY
LA  - eng
PT  - Journal Article
DEP - 20110205
PL  - United States
TA  - Urology
JT  - Urology
JID - 0366151
SB  - IM
CIN - Urology. 2011 Apr;77(4):859; author reply 859-60. PMID: 21477711
MH  - Adult
MH  - Aged
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm, Residual/diagnosis/pathology
MH  - Reoperation
MH  - Tumor Burden
MH  - Urethra
MH  - Urinary Bladder Neoplasms/*pathology/*surgery
MH  - Urologic Surgical Procedures
EDAT- 2011/02/08 06:00
MHDA- 2011/07/05 06:00
CRDT- 2011/02/08 06:00
PHST- 2010/08/04 00:00 [received]
PHST- 2010/09/28 00:00 [revised]
PHST- 2010/09/28 00:00 [accepted]
PHST- 2011/02/08 06:00 [entrez]
PHST- 2011/02/08 06:00 [pubmed]
PHST- 2011/07/05 06:00 [medline]
AID - S0090-4295(10)01947-3 [pii]
AID - 10.1016/j.urology.2010.09.061 [doi]
PST - ppublish
SO  - Urology. 2011 Apr;77(4):855-9. doi: 10.1016/j.urology.2010.09.061. Epub 2011 Feb 
      5.