PMID- 21298680
OWN - NLM
STAT- MEDLINE
DCOM- 20110907
LR  - 20171116
IS  - 1521-4028 (Electronic)
IS  - 0233-111X (Linking)
VI  - 51
IP  - 3
DP  - 2011 Jun
TI  - Isolation, characterization and identification of a Paracoccus sp. 
      2-haloacid-degrading bacterium from the marine sponge Hymeniacidon perlevis.
PG  - 318-24
LID - 10.1002/jobm.201000205 [doi]
AB  - A 2-haloacid dehalogenase-producing bacterium, designated DEH99, was isolated 
      from the marine sponge Hymeniacidon perlevis using a modified enrichment medium 
      and a pH indicator method. DEH99 could degrade only half of the racemic mixture 
      2-chloropropionic acid (2-CPA) in the medium. The dehalogenase of DEH99 was 
      further determined to be a (S)-2-haloacid dehalogenase, which can degrade 2-CPA, 
      2-bromopropionic acid (2-BPA), and iodoacetic acid. The gene encoding the 
      (S)-2-haloacid dehalogenase was partially sequenced and classified into the Group 
      II family. The 2-haloacid dehalogenase showed the highest sequence similarity 
      (77% with 21% query coverage) to the haloacid dehalogenase (dhlB) gene of 
      Xanthobacter autotrophicus. A phylogenetic analysis of the 16S rDNA sequence 
      demonstrated that the isolate DEH99 is a member of the genus Paracoccus. To our 
      knowledge, this is the first report detailing the isolation of a strain of genus 
      Paracoccus having 2-haloacid dehalogenase activity from marine sponges.
CI  - Copyright (c) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Huang, Jianyu
AU  - Huang J
AD  - Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, 
      Chinese Academy of Sciences, Dalian, China.
FAU - Xin, Yanjuan
AU  - Xin Y
FAU - Zhang, Wei
AU  - Zhang W
LA  - eng
SI  - GENBANK/GQ996493
PT  - Journal Article
DEP - 20110207
PL  - Germany
TA  - J Basic Microbiol
JT  - Journal of basic microbiology
JID - 8503885
RN  - 0 (Culture Media)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Hydrocarbons, Chlorinated)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Propionates)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - ADV1WUE1NB (2-chloropropionic acid)
RN  - EC 3.- (Hydrolases)
RN  - EC 3.8.1.2 (2-haloacid dehalogenase)
RN  - UE0XVY2DT6 (2-bromopropionic acid)
RN  - WF5188V710 (Iodoacetic Acid)
SB  - IM
MH  - Animals
MH  - Cluster Analysis
MH  - Culture Media/chemistry
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Hydrocarbons, Chlorinated
MH  - Hydrogen-Ion Concentration
MH  - Hydrolases/genetics/*metabolism
MH  - Indicators and Reagents
MH  - Iodoacetic Acid/metabolism
MH  - Molecular Sequence Data
MH  - Paracoccus/*classification/genetics/*isolation & purification
MH  - Phylogeny
MH  - Porifera/*microbiology
MH  - Propionates/metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Substrate Specificity
EDAT- 2011/02/08 06:00
MHDA- 2011/09/08 06:00
CRDT- 2011/02/08 06:00
PHST- 2010/05/29 00:00 [received]
PHST- 2010/10/12 00:00 [accepted]
PHST- 2011/02/08 06:00 [entrez]
PHST- 2011/02/08 06:00 [pubmed]
PHST- 2011/09/08 06:00 [medline]
AID - 10.1002/jobm.201000205 [doi]
PST - ppublish
SO  - J Basic Microbiol. 2011 Jun;51(3):318-24. doi: 10.1002/jobm.201000205. Epub 2011 
      Feb 7.