PMID- 21299527
OWN - NLM
STAT- MEDLINE
DCOM- 20110610
LR  - 20191210
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 77
IP  - 3
DP  - 2011 Mar
TI  - Challenging the golden standard in defining donor-specific antibodies: does the 
      solid phase assay meet the expectations?
PG  - 225-8
LID - 10.1111/j.1399-0039.2010.01608.x [doi]
AB  - The purpose of the study was to compare three different methods defining 
      donor-specific antibodies (DSA): complement-dependent cytotoxicity (CDC), the 
      flow cytometry method (FCM), and a special for that purpose commercially 
      available Luminex-based solid phase assay (SPA). A panel of human monoclonal 
      antibodies (HuMabs) with well-defined human leukocyte antigen (HLA) specificities 
      was used as antibody source and single HLA antigen expressing cell lines (SAL) 
      were used as targets. Two methods yielded identical results (CDC and FCM). 
      However, the SPA, the method by which solubilized HLA molecules from the SAL are 
      captured by microspheres, showed two additional reactions which could not be 
      explained, neither by the epitope recognized by the HuMab nor by the widely 
      accepted sensitivity of the SPA methodology. These unexplained results suggest 
      that by capturing solubilized HLA molecules on microspheres, conformational 
      changes might occur. Positive results obtained by similar Luminex-based 
      microsphere methods should be therefore taken with caution and the 'recognized' 
      HLA antigens should not automatically be considered as unacceptable for 
      transplantation.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Zoet, Y M
AU  - Zoet YM
AD  - Department of Immunohaematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, The Netherlands.
FAU - Brand-Schaaf, S H
AU  - Brand-Schaaf SH
FAU - Roelen, D L
AU  - Roelen DL
FAU - Mulder, A
AU  - Mulder A
FAU - Claas, F H J
AU  - Claas FH
FAU - Doxiadis, I I N
AU  - Doxiadis II
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Antibodies)
RN  - 0 (Antibodies, Monoclonal)
SB  - IM
MH  - Antibodies/*blood/chemistry/*isolation & purification
MH  - Antibodies, Monoclonal/immunology
MH  - Antibody Specificity
MH  - *Blood Donors
MH  - Cell Line
MH  - Cytotoxicity Tests, Immunologic/methods/standards
MH  - Flow Cytometry/methods
MH  - Histocompatibility Testing/methods/standards
MH  - Humans
MH  - K562 Cells
MH  - Reference Standards
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
MH  - Solid Phase Extraction/*standards/*statistics & numerical data
EDAT- 2011/02/09 06:00
MHDA- 2011/06/11 06:00
CRDT- 2011/02/09 06:00
PHST- 2011/02/09 06:00 [entrez]
PHST- 2011/02/09 06:00 [pubmed]
PHST- 2011/06/11 06:00 [medline]
AID - 10.1111/j.1399-0039.2010.01608.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2011 Mar;77(3):225-8. doi: 10.1111/j.1399-0039.2010.01608.x.