PMID- 21299532
OWN - NLM
STAT- MEDLINE
DCOM- 20110610
LR  - 20191210
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 77
IP  - 3
DP  - 2011 Mar
TI  - Resolution of HLA class I sequence-based typing ambiguities by group-specific 
      sequencing primers.
PG  - 247-50
LID - 10.1111/j.1399-0039.2010.01616.x [doi]
AB  - The increasing demand for allele-level human leukocyte antigen (HLA) typing has 
      led the sequence-based typing (SBT) to become the preferred method. In turn, the 
      steady increase in the number of HLA alleles driven by the adoption of SBT as the 
      ultimate typing method leads to the ever increasing number of cis/trans 
      ambiguities. Over the last few years, additional sequencing with the commercially 
      available group-specific sequencing primers (GSSPs) has replaced 
      sequence-specific primer-polymerase chain reaction and group-specific 
      amplification as the means of resolving cis/trans ambiguities in many 
      laboratories. Here we summarize our 3-year experience in designing and utilizing 
      GSSPs for resolution of HLA class I ambiguities. The panel of GSSPs used in our 
      laboratory includes 14 primers for HLA-A, 18 for HLA-B, and 13 primers for HLA-C. 
      The panel resolves 99.9% of all ambiguities.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Lebedeva, T V
AU  - Lebedeva TV
AD  - HLA Laboratory, American Red Cross Blood Services, New England Region, Dedham, MA 
      02026, USA. LebedevaT@usa.redcross.org
FAU - Mastromarino, S A
AU  - Mastromarino SA
FAU - Lee, E
AU  - Lee E
FAU - Ohashi, M
AU  - Ohashi M
FAU - Alosco, S M
AU  - Alosco SM
FAU - Yu, N
AU  - Yu N
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes, HLA)
SB  - IM
MH  - Alleles
MH  - Base Sequence
MH  - *DNA Primers/genetics
MH  - DNA Probes, HLA/analysis/genetics
MH  - False Positive Reactions
MH  - Genes, MHC Class I/*genetics
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Molecular Sequence Data
MH  - Retrospective Studies
MH  - Sequence Analysis, DNA/methods/*standards
MH  - Sequence Homology
MH  - Software
MH  - Substrate Specificity/genetics/immunology
EDAT- 2011/02/09 06:00
MHDA- 2011/06/11 06:00
CRDT- 2011/02/09 06:00
PHST- 2011/02/09 06:00 [entrez]
PHST- 2011/02/09 06:00 [pubmed]
PHST- 2011/06/11 06:00 [medline]
AID - 10.1111/j.1399-0039.2010.01616.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2011 Mar;77(3):247-50. doi: 10.1111/j.1399-0039.2010.01616.x.