PMID- 21299582
OWN - NLM
STAT- MEDLINE
DCOM- 20110707
LR  - 20161020
IS  - 1939-165X (Electronic)
IS  - 0275-6382 (Linking)
VI  - 40
IP  - 1
DP  - 2011 Mar
TI  - Monitoring unfractionated heparin therapy in dogs by measuring thrombin 
      generation.
PG  - 24-31
LID - 10.1111/j.1939-165X.2011.00282.x [doi]
AB  - BACKGROUND: The calibrated automated thrombogram (CAT), an assay that permits 
      measurement of thrombin generation in plasma, may be useful in studying 
      hemostatic disorders and anticoagulant therapy in animals. OBJECTIVES: The aims 
      of the study were to measure thrombin generation in healthy Beagle dogs and to 
      evaluate the potential use of the CAT assay for monitoring therapy with 
      unfractionated heparin (UFH). METHODS: Individual platelet-poor plasma samples 
      and a plasma pool from 20 healthy adult Beagles were prepared. Serial UFH plasma 
      dilutions were used to establish an in vitro heparin-sensitivity curve. The 
      pharmacodynamic effects of heparin in vivo were evaluated in Beagles using the 
      CAT assay to measure thrombin generation with tissue factor at a concentration of 
      5 pM for initiation. RESULTS: In healthy Beagles, the range of endogenous 
      thrombin potential (ETP) was 238.7-414.0 nM/min (mean +/- SD, 340.4 +/- 63.1 nM/min). 
      ETP intra-assay and interassay variations were 7.1% and 12.9%, respectively. In 
      vitro, a UFH concentration >/=0.4 U/mL resulted in total inhibition of thrombin 
      generation. In vivo, the maximal effect of UFH on ETP was observed at 170 +/- 36 
      minutes (range, 120-210 minutes) and resulted in a decrease in ETP of 38.5 +/- 7.8% 
      (range, 26.5-50.3%). In 210-420 minutes, ETP returned to baseline in 5 dogs. 
      CONCLUSION: Our study demonstrates that thrombin generation can be measured in 
      canine plasma and may be useful in assessing the degree of anticoagulation 
      provided by UFH.
CI  - (c)2011 American Society for Veterinary Clinical Pathology.
FAU - Allegret, Virginie
AU  - Allegret V
AD  - Charles River Laboratories, Senneville, Canada.
FAU - Dunn, Marilyn
AU  - Dunn M
FAU - Bedard, Christian
AU  - Bedard C
LA  - eng
PT  - Journal Article
DEP - 20110207
PL  - United States
TA  - Vet Clin Pathol
JT  - Veterinary clinical pathology
JID - 9880575
RN  - 0 (Anticoagulants)
RN  - 9005-49-6 (Heparin)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Animals
MH  - Anticoagulants/*therapeutic use
MH  - Blood Coagulation Tests/veterinary
MH  - Dog Diseases/blood/*drug therapy
MH  - Dogs
MH  - Hemostatic Disorders/blood/drug therapy/*veterinary
MH  - Heparin/*therapeutic use
MH  - Partial Thromboplastin Time/veterinary
MH  - Sensitivity and Specificity
MH  - Thrombin/*analysis
EDAT- 2011/02/09 06:00
MHDA- 2011/07/08 06:00
CRDT- 2011/02/09 06:00
PHST- 2011/02/09 06:00 [entrez]
PHST- 2011/02/09 06:00 [pubmed]
PHST- 2011/07/08 06:00 [medline]
AID - 10.1111/j.1939-165X.2011.00282.x [doi]
PST - ppublish
SO  - Vet Clin Pathol. 2011 Mar;40(1):24-31. doi: 10.1111/j.1939-165X.2011.00282.x. 
      Epub 2011 Feb 7.