PMID- 21300303
OWN - NLM
STAT- MEDLINE
DCOM- 20110602
LR  - 20161125
IS  - 1532-8414 (Electronic)
IS  - 1071-9164 (Linking)
VI  - 17
IP  - 2
DP  - 2011 Feb
TI  - The L-arginine-asymmetric dimethylarginine ratio is strongly related to the 
      severity of chronic heart failure. No effects of exercise training.
PG  - 135-42
LID - 10.1016/j.cardfail.2010.09.003 [doi]
AB  - BACKGROUND: The aim of this study was to relate levels of asymmetric 
      dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide (NO) synthase, 
      L-arginine, the substrate for NO generation, and radical oxygen species (ROS) 
      formation to severity of chronic heart failure. The effect of 4 months' 
      group-based exercise training was further investigated. METHOD AND RESULTS: 
      Eighty patients, aged 45-85 years with New York Heart Association (NYHA) 
      functional class II-IIIb, all on optimal medical treatment, were included. A 
      6-minute walking test and a bicycle exercise test were performed, and fasting 
      blood samples were collected for determination of N-terminal pro-brain 
      natriuretic peptide (NT-proBNP), L-arginine, ADMA, and ROS generation in 
      circulating leukocytes. ADMA levels were significantly higher in patients in NYHA 
      functional class III versus II (P = .024), and the L-arginine-ADMA ratio was 
      significantly lower (P = .005). After adjustment for covariates, L-arginine-ADMA 
      ratio was associated with 6-minute walking distance (P = .004), exercise capacity 
      (P = .026), and inversely with NT-proBNP (P = .015). Stimulated levels of 
      peroxynitrite on monocytes were inversely related to left ventricular ejection 
      fraction (P = .005). No effect of 4 weeks' exercise training on the measured 
      variables was obtained. CONCLUSIONS: The strong relationship seen between 
      L-arginine-ADMA ratio, ROS formation in leukocytes, and severity of chronic heart 
      failure contributes to increased knowledge of endothelial dysfunction related to 
      the NO pathway in such patients.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Seljeflot, Ingebjorg
AU  - Seljeflot I
AD  - Center for Clinical Heart Research, Department of Cardiology, Oslo University 
      Hospital Ulleval, Oslo, Norway. ingebjorg.seljeflot@uus.no
FAU - Nilsson, Birgitta Blakstad
AU  - Nilsson BB
FAU - Westheim, Arne S
AU  - Westheim AS
FAU - Bratseth, Vibeke
AU  - Bratseth V
FAU - Arnesen, Harald
AU  - Arnesen H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101028
PL  - United States
TA  - J Card Fail
JT  - Journal of cardiac failure
JID - 9442138
RN  - 0 (Peptide Fragments)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (pro-brain natriuretic peptide (1-76))
RN  - 114471-18-0 (Natriuretic Peptide, Brain)
RN  - 63CV1GEK3Y (N,N-dimethylarginine)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Arginine/*analogs & derivatives/*blood/metabolism
MH  - Endothelium, Vascular
MH  - *Exercise Therapy
MH  - Exercise Tolerance
MH  - Female
MH  - Flow Cytometry
MH  - Health Status Indicators
MH  - Heart Failure/drug therapy/enzymology/*therapy
MH  - Humans
MH  - Leukocytes
MH  - Male
MH  - Middle Aged
MH  - Natriuretic Peptide, Brain/*blood
MH  - Nitric Oxide Synthase/antagonists & inhibitors
MH  - Peptide Fragments/*blood
MH  - Reactive Oxygen Species/metabolism
MH  - Severity of Illness Index
MH  - Stroke Volume
MH  - Treatment Outcome
MH  - Ventricular Function, Left
EDAT- 2011/02/09 06:00
MHDA- 2011/06/03 06:00
CRDT- 2011/02/09 06:00
PHST- 2010/04/12 00:00 [received]
PHST- 2010/08/20 00:00 [revised]
PHST- 2010/09/24 00:00 [accepted]
PHST- 2011/02/09 06:00 [entrez]
PHST- 2011/02/09 06:00 [pubmed]
PHST- 2011/06/03 06:00 [medline]
AID - S1071-9164(10)01127-9 [pii]
AID - 10.1016/j.cardfail.2010.09.003 [doi]
PST - ppublish
SO  - J Card Fail. 2011 Feb;17(2):135-42. doi: 10.1016/j.cardfail.2010.09.003. Epub 
      2010 Oct 28.