PMID- 21302285
OWN - NLM
STAT- MEDLINE
DCOM- 20111115
LR  - 20120403
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 226
IP  - 11
DP  - 2011 Nov
TI  - Bone marrow-derived stem cells contribute skin regeneration in skin and soft 
      tissue expansion.
PG  - 2834-40
LID - 10.1002/jcp.22634 [doi]
AB  - Skin and soft tissue expansion stimulates the proliferation of skin epidermal 
      basal cells and increase the dermal collagen deposition and angiogenesis. To 
      explore the contribution of bone marrow-derived stem cells (BMSCs) to the 
      generation of "new" skin during the expansion, we used a chimeric mouse model in 
      which the donor C57BL mice were engrafted with the bone marrow of enhanced green 
      fluorescent protein (EGFP) transgenic mice. BMSCs were collected from the tibia 
      and femur of EGFP(+) transgenic mice, and then injected into normal C57BL mice 
      via the tail vein (chimeric mice). Skin was obtained at different times (days 0, 
      7, 14, 21, 28, and 35). Skin stromal-derived factor-1 (SDF-1) expression was 
      evaluated. The number, distribution, and phenotype changes of EGFP(+) cells in 
      the skin were also evaluated by means of fluorescent microscopy. EGFP(+) cells 
      were present stably in the normal skin. The number of EGFP(+) cells of the Group 
      A mice changed with the tension, and reached the peak on day 21(17.1 +/- 6.7%), as 
      compared with either Group B (5.5 +/- 1.0%) or Group C (5.1 +/- 0.9%). The SDF-1 
      expression in the expanded skin was significant increased ( approximately 11-fold, P < 0.01) 
      compared to non-expanded skin on day 21. Immunofluorescence showed EGFP(+) cells 
      were converted into vascular endothelial cells, epidermal cells, and 
      spindle-shaped dermal fibroblasts. Strain can promote the expression of SDF-1 and 
      facilitate the differentiation and proliferation of BMSCs in the expanded skin.
CI  - Copyright (c) 2011 Wiley-Liss, Inc.
FAU - Li, Chichi
AU  - Li C
AD  - Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical 
      University, Xi'an, Shanxi Province, China.
FAU - Zheng, Yan
AU  - Zheng Y
FAU - Wang, Xiaoyan
AU  - Wang X
FAU - Xia, Wei
AU  - Xia W
FAU - Gao, Hongying
AU  - Gao H
FAU - Li, Dong
AU  - Li D
FAU - Ma, Xianjie
AU  - Ma X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Cxcl12 protein, mouse)
RN  - 0 (Silicones)
SB  - IM
EIN - J Cell Physiol. 2012 Jun;227(6):2805. Wang, Xiaoyan [removed]
MH  - Animals
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Cell Differentiation
MH  - Chemokine CXCL12/biosynthesis
MH  - Chimera/metabolism
MH  - Endothelial Cells/metabolism
MH  - Fibroblasts/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Prostheses and Implants
MH  - *Regeneration
MH  - Silicones/metabolism
MH  - Skin/cytology
MH  - *Skin Physiological Phenomena
MH  - Stem Cells/*physiology
MH  - *Tissue Expansion
EDAT- 2011/02/09 06:00
MHDA- 2011/11/16 06:00
CRDT- 2011/02/09 06:00
PHST- 2011/02/09 06:00 [entrez]
PHST- 2011/02/09 06:00 [pubmed]
PHST- 2011/11/16 06:00 [medline]
AID - 10.1002/jcp.22634 [doi]
PST - ppublish
SO  - J Cell Physiol. 2011 Nov;226(11):2834-40. doi: 10.1002/jcp.22634.