PMID- 21302433
OWN - NLM
STAT- MEDLINE
DCOM- 20110929
LR  - 20131121
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 41
IP  - 5
DP  - 2010 Sep
TI  - [Quercetin permeability across blood-brain barrier and its effect on the 
      viability of U251 cells].
PG  - 751-4, 759
AB  - OBJECTIVE: To investigate the permeability of quercetin across blood-brain 
      barrier (BBB) and its impact on the proliferation and apoptosis of U251 cells. 
      METHODS: The BBB model was established through culture of primary brain 
      microvessel endothelial cells (BMVEC) and primary astroglia cells (AC), which was 
      confirmed by transmission electron microscopy (TEM) and trans epithelial electric 
      resistance (TEER). High pressure liquid chromatography (HPLC) was performed to 
      determine quercetin permeability across BBB. U251 cells were exposed to 
      quercetin. MTT assay, terminal deoxynucleotidyl transferase-mediated dUTP nick 
      end labeling (TUNEL), single-cell gel electrophoresis (SCGE) and flow cytometry 
      (FCM) were performed to analyze cell proliferation, apoptosis, DNA damage and 
      cell cycle distribution. RESULTS: The BBB was constructed successfully. Up to 
      65.54% of quercetin permeated across the BBB. Quercetin attenuated the 
      proliferation of U251 and induced apoptosis. The FCM revealed that the U251 cells 
      were inhibited at the G2/M point. CONCLUSION: Quercetin can permeate across the 
      BBB effectively, restraining the proliferation of U251 cells and inducing 
      apoptosis.
FAU - Ren, Si-chong
AU  - Ren SC
AD  - Department of Biochemistry and Molecular Biology, West China School of 
      Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
FAU - Suo, Qi-feng
AU  - Suo QF
FAU - Du, Wen-ting
AU  - Du WT
FAU - Pan, Hong
AU  - Pan H
FAU - Yang, Ming-ming
AU  - Yang MM
FAU - Wang, Ruo-han
AU  - Wang RH
FAU - Liu, Ji
AU  - Liu J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Antineoplastic Agents)
RN  - 9IKM0I5T1E (Quercetin)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antineoplastic Agents/pharmacokinetics/pharmacology
MH  - Apoptosis/drug effects
MH  - Blood-Brain Barrier/*drug effects
MH  - Brain Neoplasms/*pathology
MH  - Cell Line, Tumor
MH  - Cell Membrane Permeability/*drug effects
MH  - Cell Survival/drug effects
MH  - Endothelial Cells/metabolism
MH  - Glioma/*pathology
MH  - Humans
MH  - Models, Biological
MH  - Quercetin/*pharmacokinetics/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2011/02/10 06:00
MHDA- 2011/10/01 06:00
CRDT- 2011/02/10 06:00
PHST- 2011/02/10 06:00 [entrez]
PHST- 2011/02/10 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2010 Sep;41(5):751-4, 759.