PMID- 21302491
OWN - NLM
STAT- MEDLINE
DCOM- 20130204
LR  - 20161018
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 30
IP  - 12
DP  - 2010 Dec
TI  - [Effects of xiongshao capsule combined with ischemic postconditioning on monocyte 
      chemoattractant protein-1 and tumor necrosis factor-alpha in rat myocardium with 
      ischemic reperfusion injury].
PG  - 1279-83
AB  - OBJECTIVE: To investigate effect of Xiongshao Capsule (XSC) combined with 
      ischemic postconditioning (IPoC) on tumor necrosis factor-alpha (TNF-alpha) and 
      monocyte chemoattractant protein-1 (MCP-1) contents as well as inflammatory cell 
      infiltration (ICI) in myocardium of rat with ischemic reperfusion (I/R) injury. 
      METHODS: Seventy-five Sprague-Dawley rats were equally randomized into 5 groups, 
      the sham-operated group (A), the I/R group (B), the IPoC group (C), the 
      fosinopril sodium plus IPoC group (D), and the XSC plus IPoC group (E). Excepting 
      rats in Group A, all animals received I/R injury through a 30-min occlusion of 
      left anterior descending artery followed by 1-h reperfusion. Additionally, IPoC 
      (3 cycles of 10 s reperfusion/10 s of ischemia) was applied on rats in Group C 
      before 1 h of reperfusion; while rats in Groups D and E were pretreated for 14 
      days with 0.9 mg/kg fosinopril sodium and 0.135 g/kg XSC respectively via 
      gastrogavage, and the I/R injury with IPoC applied 2 h after the final gavage. 
      Serum creatine kinase-MB (CK-MB) and cardiac troponin T (cTnT) levels were 
      detected by colorimetric method, myocardial infarction size was measured by nitro 
      blue tetrazolium chloride (NBT) staining, MCP-1 and TNF-alpha contents in 
      myocardial tissue were examined by enzyme-linked immunosorbent assay (ELISA), and 
      ICI was detected by HE staining. RESULTS: Compared with Group B, myocardial 
      enzymes and infarction size were significantly decreased (P<0.01), contents of 
      MCP-1, TNF-alpha and ICI in myocardial tissue were significantly decreased 
      (P<0.05, P<0.01) in Group C. Compared with Group C, further reduced infarction 
      size and release of myocardial enzyme CK-MB (P<0.01) were seen in Group E, and 
      contents of MCP-1 and TNF-alpha as well as ICI in myocardial tissue in Group E 
      were also significantly lower (P<0.05, P<0.01). CONCLUSION: XSC could enhance the 
      protective effect of IPoC on rat with myocardial I/R injury, and the mechanism 
      may be related to its inhibition on MCP-1 and TNF-alpha expressions as well as 
      ICI suppression.
FAU - Zhang, Da-wu
AU  - Zhang DW
AD  - Department of Cardiology, Xiyuan Hospital, China Academy of Chinese Medical 
      Sciences, Beijing 100091.
FAU - Zhang, Lei
AU  - Zhang L
FAU - Liu, Jian-gang
AU  - Liu JG
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (xiongshao)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*metabolism
MH  - Drugs, Chinese Herbal/*pharmacology/therapeutic use
MH  - Female
MH  - Ischemic Postconditioning/*methods
MH  - Male
MH  - Myocardial Ischemia/physiopathology
MH  - Myocardial Reperfusion Injury/metabolism/*prevention & control
MH  - Myocardium/metabolism
MH  - Phytotherapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2011/02/10 06:00
MHDA- 2013/02/05 06:00
CRDT- 2011/02/10 06:00
PHST- 2011/02/10 06:00 [entrez]
PHST- 2011/02/10 06:00 [pubmed]
PHST- 2013/02/05 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Dec;30(12):1279-83.