PMID- 21309693
OWN - NLM
STAT- MEDLINE
DCOM- 20110929
LR  - 20161125
IS  - 1521-0464 (Electronic)
IS  - 1071-7544 (Linking)
VI  - 18
IP  - 5
DP  - 2011 Jul
TI  - Visualization of Connexin 43-positive cells of glioma and the periglioma zone by 
      means of intravenously injected monoclonal antibodies.
PG  - 331-7
LID - 10.3109/10717544.2010.549527 [doi]
AB  - The selectivity of monoclonal antibodies against the E2 extracellular fragment of 
      connexin 43 (Cx43) for a glioma focus was studied in in vivo experiments on 
      animals with intracranial C6 glioma. Antibodies labeled with two alternative 
      labels, the radioisotope (125)I and the fluorophore Alexa 660, were intravenously 
      injected to rats with 18-day gliomas. Seventy-two hours after injection, 
      (125)I-labeled antibodies accumulated in the hemisphere where the glioma was 
      located to a concentration of 0.27 +/- 0.01% of the injected dose per gram of wet 
      weight, which exceeded their accumulation in the liver, spleen, and other organs. 
      Fluorescent-labeled antibodies against the Cx43 fragment E2 specifically 
      visualized cells in the peritumoral astroglial bank (a zone of active invasion of 
      glioma cells). Double immunofluorescent visualization using antibodies against 
      the Cx43 fragment E2 and glial fibrillar acidic protein (GFAP) showed that only a 
      small proportion of the cells that bound the antibodies injected into the blood 
      circulation were reactive astrocytes, whereas most of these cells were 
      GFAP-negative and morphologically corresponded to astroblasts. These results 
      suggest that antibodies against the extracellular Cx43 fragment E2 can be used 
      for targeted transport of diagnostic and therapeutic drugs to the peritumoral 
      invasion zone of high-grade gliomas.
FAU - Baklaushev, Vladimir P
AU  - Baklaushev VP
AD  - Pirogov Russian State Medical University, Chair of Medicinal Nanobiotechnology, 
      Moscow, Russia. serpoff@gmail.com
FAU - Yusubalieva, Gaukhar M
AU  - Yusubalieva GM
FAU - Tsitrin, Eugene B
AU  - Tsitrin EB
FAU - Gurina, Olga I
AU  - Gurina OI
FAU - Grinenko, Nadezhda Ph
AU  - Grinenko NP
FAU - Victorov, Ilya V
AU  - Victorov IV
FAU - Chekhonin, Vladimir P
AU  - Chekhonin VP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20110210
PL  - England
TA  - Drug Deliv
JT  - Drug delivery
JID - 9417471
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Connexin 43)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Iodine Radioisotopes)
SB  - IM
MH  - Animals
MH  - *Antibodies, Monoclonal/pharmacokinetics
MH  - Astrocytes/metabolism
MH  - Connexin 43/*metabolism
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Glioma/*diagnostic imaging
MH  - Injections, Intravenous
MH  - Iodine Radioisotopes
MH  - Radionuclide Imaging
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Distribution
EDAT- 2011/02/12 06:00
MHDA- 2011/10/01 06:00
CRDT- 2011/02/12 06:00
PHST- 2011/02/12 06:00 [entrez]
PHST- 2011/02/12 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
AID - 10.3109/10717544.2010.549527 [doi]
PST - ppublish
SO  - Drug Deliv. 2011 Jul;18(5):331-7. doi: 10.3109/10717544.2010.549527. Epub 2011 
      Feb 10.