PMID- 21310555
OWN - NLM
STAT- MEDLINE
DCOM- 20120508
LR  - 20220408
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 150
IP  - 1-2
DP  - 2011 May 12
TI  - Genetic and immunobiological diversities of porcine reproductive and respiratory 
      syndrome genotype I strains.
PG  - 49-62
LID - 10.1016/j.vetmic.2011.01.008 [doi]
AB  - Genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) 
      has been based on ORF5/GP5 and ORF7/N protein variations. Complete viral genome 
      studies are limited and focused on a single or a few set of strains. Moreover, 
      there is a general tendency to extrapolate results obtained from a single isolate 
      to the overall PRRSV population. In the present study, six genotype-I isolates of 
      PRRSV were sequenced from ORF1a to ORF7. Phylogenetic comparisons and the 
      variability degree of known linear B-epitopes were done considering other 
      available full-length genotype-I sequences. Cytokine induction of all strains was 
      also evaluated in different cellular systems. Non structural protein 2 (nsp2) was 
      the most variable part of the virus with 2 out of 6 strains harboring a 74 aa 
      deletion. Deletions were also found in ORF3 and ORF4. Phylogenetic analyses 
      showed that isolates could be grouped differently depending on the ORF examined 
      and the highest similarity with the full genome cluster was found for the nsp9. 
      Interestingly, most of predicted linear B-epitopes in the literature, 
      particularly in nsp2 and GP4 regions, were found deleted or varied in some of our 
      isolates. Moreover, 4 strains, those with deletions in nsp2, induced TNF-alpha and 3 
      induced IL-10. These results underline the high genetic diversity of PRRSV mainly 
      in nsp1, nsp2 and ORFs 3 and 4. This variability also affects most of the known 
      linear B-epitopes of the virus. Accordingly, different PRRSV strains might have 
      substantially different immunobiological properties. These data can contribute to 
      the understanding of PRRSV complexity.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Darwich, Laila
AU  - Darwich L
AD  - Departament de Sanitat i Anatomia Animals, Universitat Autonoma de Barcelona, 
      08193 Bellaterra, Spain. laila.darwich@uab.cat
FAU - Gimeno, Mariona
AU  - Gimeno M
FAU - Sibila, Marina
AU  - Sibila M
FAU - Diaz, Ivan
AU  - Diaz I
FAU - de la Torre, Eugenia
AU  - de la Torre E
FAU - Dotti, Silvia
AU  - Dotti S
FAU - Kuzemtseva, Liudmila
AU  - Kuzemtseva L
FAU - Martin, Margarita
AU  - Martin M
FAU - Pujols, Joan
AU  - Pujols J
FAU - Mateu, Enric
AU  - Mateu E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110115
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Cytokines)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cells, Cultured
MH  - Cluster Analysis
MH  - Cytokines/immunology
MH  - Epitopes, B-Lymphocyte/genetics
MH  - *Genetic Variation
MH  - Genome, Viral
MH  - Genotype
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - *Phylogeny
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*immunology
MH  - Sequence Deletion
MH  - Swine
MH  - Viral Nonstructural Proteins/genetics
EDAT- 2011/02/12 06:00
MHDA- 2012/05/09 06:00
CRDT- 2011/02/12 06:00
PHST- 2010/06/22 00:00 [received]
PHST- 2010/12/30 00:00 [revised]
PHST- 2011/01/10 00:00 [accepted]
PHST- 2011/02/12 06:00 [entrez]
PHST- 2011/02/12 06:00 [pubmed]
PHST- 2012/05/09 06:00 [medline]
AID - S0378-1135(11)00012-5 [pii]
AID - 10.1016/j.vetmic.2011.01.008 [doi]
PST - ppublish
SO  - Vet Microbiol. 2011 May 12;150(1-2):49-62. doi: 10.1016/j.vetmic.2011.01.008. 
      Epub 2011 Jan 15.