PMID- 21314837
OWN - NLM
STAT- MEDLINE
DCOM- 20111004
LR  - 20211020
IS  - 1525-1594 (Electronic)
IS  - 0160-564X (Print)
IS  - 0160-564X (Linking)
VI  - 35
IP  - 6
DP  - 2011 Jun
TI  - What blood temperature for an ex vivo extracorporeal circuit?
PG  - 593-601
LID - 10.1111/j.1525-1594.2010.01147.x [doi]
AB  - Ex vivo circuits are commonly used to evaluate biomaterials or devices used for 
      extracorporeal blood purification. However, various aspects of the ex vivo 
      circuit, apart from the circuit materials, may affect inflammation and 
      coagulation. One such aspect is temperature. The aim of this study was to 
      evaluate the influence of different blood temperature conditions on inflammation 
      parameters in an ex vivo circuit. Blood was collected from 20 healthy volunteers 
      and run through three different experimental conditions for 4 h: a miniaturized 
      ex vivo extracorporeal circuit equipped with a blood warmer set to 37 degrees C, the same 
      circuit without the warmer (23 degrees C), and a tube placed in an incubator at 37 degrees C (no 
      circuit). We measured the granulocyte macrophage colony-stimulating factor, the 
      tumor necrosis factor, and the interleukin (IL)-1beta, IL-6, IL-8, and IL-10 
      concentrations at baseline, 15, 60, 120, and 240 min. Human leukocyte antigen 
      (HLA)-DR, CD11b, CD11a, CD62L, tumor necrosis factor alpha converting enzyme, 
      annexin V expression, and NFkB DNA binding were measured in monocytes and 
      polymorphonuclear neutrophils (PMNs) using flow cytometry at baseline, 120 min, 
      and 240 min. While cytokine production over time was very slight at room 
      temperature, levels increased by more than 100-fold in the two heated conditions. 
      Differences in the expression of some surface markers were also observed between 
      the room temperature circuit and the two heated conditions (CD11b PMN, P < 
      0.0001; HLA-DR Mono, P=0.0019; and CD11a PMN, P<0.0001). Evolution of annexin V 
      expression was also different over time between the three groups (P=0.0178 for 
      monocytes and P=0.0011 for PMNs). A trend for a greater NFkB DNA binding was 
      observed in the heated conditions. Thus, for ex vivo studies using extracorporeal 
      circuits, heating blood to maintain body temperature results in significant 
      activation of inflammatory cells while hypothermia (room temperature) seems to 
      suppress the leukocyte response. Both strategies may lead to erroneous 
      conclusions, possibly masking some specific effects of the device being studied. 
      Investigators in this field must be aware of the fact that blood temperature is a 
      crucial confounding parameter and the type of "background noise" they will face 
      depending on the strategy adopted.
CI  - (c) 2011, Copyright the Authors. Artificial Organs (c) 2011, International Center for 
      Artificial Organs and Transplantation and Wiley Periodicals, Inc.
FAU - Rimmele, Thomas
AU  - Rimmele T
AD  - The CRISMA (Clinical Research, Investigation, and Systems Modeling of Acute 
      Illness) Laboratory, Department of Critical Care Medicine, University of 
      Pittsburgh Medical Center, Pittsburgh, PA, USA.
FAU - Bishop, Jeffrey
AU  - Bishop J
FAU - Simon, Peter
AU  - Simon P
FAU - Carter, Melinda
AU  - Carter M
FAU - Kong, Lan
AU  - Kong L
FAU - Lee, Minjae
AU  - Lee M
FAU - Singbartl, Kai
AU  - Singbartl K
FAU - Kellum, John A
AU  - Kellum JA
LA  - eng
GR  - R01 HL080926/HL/NHLBI NIH HHS/United States
GR  - R01 HL080926-05/HL/NHLBI NIH HHS/United States
GR  - 1R01HL080926/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110214
PL  - United States
TA  - Artif Organs
JT  - Artificial organs
JID - 7802778
RN  - 0 (Cytokines)
RN  - 0 (Interleukins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Adult
MH  - Body Temperature
MH  - Cytokines/*blood
MH  - Equipment Design
MH  - Extracorporeal Circulation/instrumentation/*methods
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/blood
MH  - Heating
MH  - Humans
MH  - Interleukins/blood
MH  - Leukocytes/*immunology
MH  - Tumor Necrosis Factor-alpha/blood
MH  - Young Adult
PMC - PMC3224854
MID - NIHMS334496
EDAT- 2011/02/15 06:00
MHDA- 2011/10/05 06:00
PMCR- 2012/06/01
CRDT- 2011/02/15 06:00
PHST- 2011/02/15 06:00 [entrez]
PHST- 2011/02/15 06:00 [pubmed]
PHST- 2011/10/05 06:00 [medline]
PHST- 2012/06/01 00:00 [pmc-release]
AID - 10.1111/j.1525-1594.2010.01147.x [doi]
PST - ppublish
SO  - Artif Organs. 2011 Jun;35(6):593-601. doi: 10.1111/j.1525-1594.2010.01147.x. Epub 
      2011 Feb 14.