PMID- 21315019
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20110214
IS  - 1009-3419 (Print)
IS  - 1009-3419 (Linking)
VI  - 5
IP  - 1
DP  - 2002 Feb 20
TI  - [Molecular cloning of genes differentially expressed in human lung squamous cell 
      carcinoma using suppression subtractive hybridization].
PG  - 10-3
LID - 10.3779/j.issn.1009-3419.2002.01.03 [doi]
AB  - BACKGROUND: To clone and identify genes differentially expressed in human lung 
      squamous cell carcinoma (LSCC). METHODS: A subtracted cDNA library of human LSCC 
      was constructed by suppression subtracted hybridization (SSH) method. After 
      screening, the subtracted library clones representing mRNAs that were truly 
      differentially expressed in LSCC but not in its adjacent non cancerous tissues 
      were selected to identify by RT-PCR and DNA sequencing were performed. Nucleic 
      acid homology searches were performed using the BLAST program. RESULTS: By this 
      technique, 10 differentially expressed gene cDNA fragments of LSSC were obtained. 
      Two were novel and eight were already known genes. CONCLUSIONS: SSH is a useful 
      technique with high sensitivity for the detection of differential genes 
      expression in LSCC and an effective method to clone novel genes.
FAU - Shen, Chenyang
AU  - Shen C
AD  - Department of Thoracic Surgery, The People's Hospital, Peking University, Beijing 
      100044, P.R.China.
FAU - Liu, Jun
AU  - Liu J
FAU - Wang, Danlei
AU  - Wang D
FAU - Zhao, Hui
AU  - Zhao H
FAU - Jiang, Guanchao
AU  - Jiang G
FAU - Wang, Jun
AU  - Wang J
FAU - Zhang, Guoliang
AU  - Zhang G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Fei Ai Za Zhi
JT  - Zhongguo fei ai za zhi = Chinese journal of lung cancer
JID - 101126433
EDAT- 2002/02/20 00:00
MHDA- 2002/02/20 00:01
CRDT- 2011/02/15 06:00
PHST- 2011/02/15 06:00 [entrez]
PHST- 2002/02/20 00:00 [pubmed]
PHST- 2002/02/20 00:01 [medline]
AID - 10.3779/j.issn.1009-3419.2002.01.03 [doi]
PST - ppublish
SO  - Zhongguo Fei Ai Za Zhi. 2002 Feb 20;5(1):10-3. doi: 
      10.3779/j.issn.1009-3419.2002.01.03.