PMID- 21315792
OWN - NLM
STAT- MEDLINE
DCOM- 20110915
LR  - 20191210
IS  - 1638-6183 (Electronic)
IS  - 0300-9084 (Linking)
VI  - 93
IP  - 5
DP  - 2011 May
TI  - 2-Methyl-pyran-4-one-3-O-beta-D-glucopyranoside isolated from leaves of Punica 
      granatum inhibits the TNFalpha-induced cell adhesion molecules expression by blocking 
      nuclear transcription factor-kappaB (NF-kappaB).
PG  - 921-30
LID - 10.1016/j.biochi.2011.01.010 [doi]
AB  - Here, we report bioactivity-guided isolation, purification and characterization 
      of a novel compound, 2-methyl-pyran-4-one-3-O-beta-d-glucopyranoside (MPG) from the 
      leaves of Punica granatum. The structure of MPG was established on the basis of 
      its detailed spectral analyses. We demonstrated that MPG not only inhibited the 
      expression of cell adhesion molecules but also significantly blocked its 
      functional consequence, that is, the adhesion of neutrophils on human endothelial 
      cells monolayer. To elucidate the molecular mechanism of action of MPG, we showed 
      that MPG decreased the transcript levels of ICAM-1, VCAM-1 and E-selectin genes. 
      Using electrophoretic mobility shift assay (EMSA) and western blot analyses, we 
      demonstrated that MPG significantly blocked both the TNFalpha-induced translocation 
      and activation of nuclear transcription factor-kappaB (NF-kappaB). Thus, MPG could be 
      useful as a novel lead molecule for developing future anti-inflammatory agents.
CI  - Copyright (c) 2011 Elsevier Masson SAS. All rights reserved.
FAU - Balwani, Sakshi
AU  - Balwani S
AD  - Institute of Genomics & Integrative Biology (Unit of C.S.I.R.), Mall Road, Delhi 
      110 007, India.
FAU - Nandi, Debkumar
AU  - Nandi D
FAU - Jaisankar, Parasuraman
AU  - Jaisankar P
FAU - Ghosh, Balaram
AU  - Ghosh B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110217
PL  - France
TA  - Biochimie
JT  - Biochimie
JID - 1264604
RN  - 0 (2-methyl-pyran-4-one-3-O-beta-D-glucopyranoside)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytotoxins)
RN  - 0 (E-Selectin)
RN  - 0 (Glucosides)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
RN  - 0 (SELE protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Cell Adhesion Molecules/genetics/*metabolism
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cytotoxins/chemistry/isolation & purification/*pharmacology
MH  - E-Selectin/genetics/metabolism
MH  - Endothelial Cells/drug effects/metabolism
MH  - Glucosides/chemistry/isolation & purification/*pharmacology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/genetics/metabolism
MH  - Lythraceae/*chemistry
MH  - Maximum Tolerated Dose
MH  - Molecular Conformation
MH  - NF-kappa B/*antagonists & inhibitors
MH  - Neutrophils/metabolism
MH  - Plant Extracts
MH  - Plant Leaves/*chemistry
MH  - Protein Transport/drug effects
MH  - Transcription, Genetic/drug effects
MH  - Tumor Necrosis Factor-alpha/*antagonists & inhibitors
MH  - Umbilical Cord/cytology
MH  - Vascular Cell Adhesion Molecule-1/genetics/metabolism
EDAT- 2011/02/15 06:00
MHDA- 2011/09/16 06:00
CRDT- 2011/02/15 06:00
PHST- 2010/10/06 00:00 [received]
PHST- 2011/01/20 00:00 [accepted]
PHST- 2011/02/15 06:00 [entrez]
PHST- 2011/02/15 06:00 [pubmed]
PHST- 2011/09/16 06:00 [medline]
AID - S0300-9084(11)00016-2 [pii]
AID - 10.1016/j.biochi.2011.01.010 [doi]
PST - ppublish
SO  - Biochimie. 2011 May;93(5):921-30. doi: 10.1016/j.biochi.2011.01.010. Epub 2011 
      Feb 17.