PMID- 21316494
OWN - NLM
STAT- MEDLINE
DCOM- 20110816
LR  - 20131121
IS  - 1878-7568 (Electronic)
IS  - 1742-7061 (Linking)
VI  - 7
IP  - 6
DP  - 2011 Jun
TI  - A new insight into the dissociating effect of strontium on bone resorption and 
      formation.
PG  - 2593-603
LID - 10.1016/j.actbio.2011.02.013 [doi]
AB  - Calcium phosphates are widely used as biomaterials and strontium (Sr) is known to 
      have the ability to modify the bone balance towards osteosynthesis. In the 
      present study we investigated the capacity of Sr-substituted sol-gel calcium 
      phosphate to modify the expression of genes and proteins involved in 
      extracellular matrix synthesis by primary bone cells. We first determined the 
      most effective concentration of strontium using human primary bone cells. Sol-gel 
      biphasic calcium phosphate (BCP) powders were then synthesised to obtain release 
      of the optimal concentration of strontium. Finally, human osteoblasts obtained 
      from explant cultures were cultured in the presence of sol-gel BCP, 
      Sr-substituted BCP (5% Sr-substituted BCP, corresponding to a release of 
      5x10(-5)M [Sr(2+)] under the culture conditions (BCP(5%))) and medium containing 
      strontium chloride (SrCl(2)). Viability, proliferation, cell morphology, protein 
      production and protein activity were studied. We demonstrated that 5x10(-5)M 
      SrCl(2) and BCP(5%) increased the expression of type I collagen and SERPINH1 mRNA 
      and reduced the production of matrix metalloproteinases (MMP-1 and MMP-2) without 
      modifying the levels of the tissue inhibitors of MMPs (TIMPs). Thus strontium has 
      a positive effect on bone formation.
CI  - Copyright (c) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights 
      reserved.
FAU - Braux, Julien
AU  - Braux J
AD  - INSERM UMR-S 926, Universite de Reims Champagne-Ardenne, IFR53, UFR Odontologie, 
      1 Avenue du Marechal Juin, F-51095 Reims Cedex, France. 
      julien.braux@univ-reims.fr
FAU - Velard, Frederic
AU  - Velard F
FAU - Guillaume, Christine
AU  - Guillaume C
FAU - Bouthors, Sylvie
AU  - Bouthors S
FAU - Jallot, Edouard
AU  - Jallot E
FAU - Nedelec, Jean-Marie
AU  - Nedelec JM
FAU - Laurent-Maquin, Dominique
AU  - Laurent-Maquin D
FAU - Laquerriere, Patrice
AU  - Laquerriere P
LA  - eng
PT  - Journal Article
DEP - 20110226
PL  - England
TA  - Acta Biomater
JT  - Acta biomaterialia
JID - 101233144
RN  - 0 (DNA Primers)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - YZS2RPE8LE (Strontium)
SB  - IM
MH  - Base Sequence
MH  - Bone Development/*drug effects
MH  - *Bone Resorption
MH  - Cells, Cultured
MH  - DNA Primers
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Matrix Metalloproteinases/metabolism
MH  - Microscopy, Electron, Scanning
MH  - Osteoblasts/*drug effects/enzymology/ultrastructure
MH  - Polymerase Chain Reaction
MH  - Strontium/*pharmacology
EDAT- 2011/02/15 06:00
MHDA- 2011/08/17 06:00
CRDT- 2011/02/15 06:00
PHST- 2010/10/31 00:00 [received]
PHST- 2011/01/06 00:00 [revised]
PHST- 2011/02/07 00:00 [accepted]
PHST- 2011/02/15 06:00 [entrez]
PHST- 2011/02/15 06:00 [pubmed]
PHST- 2011/08/17 06:00 [medline]
AID - S1742-7061(11)00072-9 [pii]
AID - 10.1016/j.actbio.2011.02.013 [doi]
PST - ppublish
SO  - Acta Biomater. 2011 Jun;7(6):2593-603. doi: 10.1016/j.actbio.2011.02.013. Epub 
      2011 Feb 26.