PMID- 21317317
OWN - NLM
STAT- MEDLINE
DCOM- 20110606
LR  - 20240320
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Print)
IS  - 0021-9193 (Linking)
VI  - 193
IP  - 8
DP  - 2011 Apr
TI  - An Enterotoxin-Bearing Pathogenicity Island in Staphylococcus epidermidis.
PG  - 1854-62
LID - 10.1128/JB.00162-10 [doi]
AB  - Cocolonization of human mucosal surfaces causes frequent encounters between 
      various staphylococcal species, creating opportunities for the horizontal 
      acquisition of mobile genetic elements. The majority of Staphylococcus aureus 
      toxins and virulence factors are encoded on S. aureus pathogenicity islands 
      (SaPIs). Horizontal movement of SaPIs between S. aureus strains plays a role in 
      the evolution of virulent clinical isolates. Although there have been reports of 
      the production of toxic shock syndrome toxin 1 (TSST-1), enterotoxin, and other 
      superantigens by coagulase-negative staphylococci, no associated pathogenicity 
      islands have been found in the genome of Staphylococcus epidermidis, a generally 
      less virulent relative of S. aureus. We show here the first evidence of a 
      composite S. epidermidis pathogenicity island (SePI), the product of multiple 
      insertions in the genome of a clinical isolate. The taxonomic placement of S. 
      epidermidis strain FRI909 was confirmed by a number of biochemical tests and 
      multilocus sequence typing. The genome sequence of this strain was analyzed for 
      other unique gene clusters and their locations. This pathogenicity island encodes 
      and expresses staphylococcal enterotoxin C3 (SEC3) and staphylococcal 
      enterotoxin-like toxin L (SElL), as confirmed by quantitative reverse 
      transcription-PCR (qRT-PCR) and immunoblotting. We present here an initial 
      characterization of this novel pathogenicity island, and we establish that it is 
      stable, expresses enterotoxins, and is not obviously transmissible by phage 
      transduction. We also describe the genome sequence, excision, replication, and 
      packaging of a novel bacteriophage in S. epidermidis FRI909, as well as attempts 
      to mobilize the SePI element by this phage.
FAU - Madhusoodanan, Jyoti
AU  - Madhusoodanan J
AD  - Department of Oral Biology, NYS Center of Excellence in Bioinformatics and Life 
      Sciences, The State University of New York, NY, USA.
FAU - Seo, Keun Seok
AU  - Seo KS
FAU - Remortel, Brian
AU  - Remortel B
FAU - Park, Joo Youn
AU  - Park JY
FAU - Hwang, Sun Young
AU  - Hwang SY
FAU - Fox, Lawrence K
AU  - Fox LK
FAU - Park, Yong Ho
AU  - Park YH
FAU - Deobald, Claudia F
AU  - Deobald CF
FAU - Wang, Dan
AU  - Wang D
FAU - Liu, Song
AU  - Liu S
FAU - Daugherty, Sean C
AU  - Daugherty SC
FAU - Gill, Ann Lindley
AU  - Gill AL
FAU - Bohach, Gregory A
AU  - Bohach GA
FAU - Gill, Steven R
AU  - Gill SR
LA  - eng
SI  - GENBANK/AENR00000000
GR  - U54 AI057141/AI/NIAID NIH HHS/United States
GR  - P20RR016454/RR/NCRR NIH HHS/United States
GR  - P20RR15587/RR/NCRR NIH HHS/United States
GR  - P20 RR016454/RR/NCRR NIH HHS/United States
GR  - P20 RR015587/RR/NCRR NIH HHS/United States
GR  - U54AI57141/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20110211
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Enterotoxins)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Bacterial Proteins/*genetics
MH  - Bacterial Typing Techniques
MH  - DNA, Bacterial/chemistry/genetics
MH  - Enterotoxins/*genetics
MH  - Gene Expression Profiling
MH  - *Genomic Islands
MH  - Humans
MH  - Immunoblotting
MH  - Molecular Sequence Data
MH  - Multilocus Sequence Typing
MH  - Mutagenesis, Insertional
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Staphylococcal Infections/microbiology
MH  - Staphylococcus epidermidis/classification/*genetics/isolation & 
      purification/pathogenicity
MH  - Virulence Factors/*genetics
PMC - PMC3133018
EDAT- 2011/02/15 06:00
MHDA- 2011/06/07 06:00
PMCR- 2011/10/01
CRDT- 2011/02/15 06:00
PHST- 2011/02/15 06:00 [entrez]
PHST- 2011/02/15 06:00 [pubmed]
PHST- 2011/06/07 06:00 [medline]
PHST- 2011/10/01 00:00 [pmc-release]
AID - JB.00162-10 [pii]
AID - 0162-10 [pii]
AID - 10.1128/JB.00162-10 [doi]
PST - ppublish
SO  - J Bacteriol. 2011 Apr;193(8):1854-62. doi: 10.1128/JB.00162-10. Epub 2011 Feb 11.