PMID- 21318355
OWN - NLM
STAT- MEDLINE
DCOM- 20110824
LR  - 20231213
IS  - 1432-203X (Electronic)
IS  - 0721-7714 (Linking)
VI  - 30
IP  - 6
DP  - 2011 Jun
TI  - Immunogenicity of nuclear-encoded LTB:ST fusion protein from Escherichia coli 
      expressed in tobacco plants.
PG  - 1145-52
LID - 10.1007/s00299-011-1023-0 [doi]
AB  - Enterotoxigenic Escherichia coli (ETEC) is one of the main causative agents of 
      diarrhea in infants and for travelers. Inclusion of a heat-stable (ST) toxin into 
      vaccine formulations is mandatory as most ETEC strains can produce both 
      heat-labile (LT) and ST enterotoxins. In this study, a genetic fusion gene 
      encoding for an LTB:ST protein has been constructed and transferred into tobacco 
      via Agrobacterium tumefaciens-mediated transformation. Transgenic tobacco plants 
      carrying the LTB:ST gene are then subjected to GM1-ELISA revealing that the 
      LTB:ST has assembled into pentamers and displays antigenic determinants from both 
      LTB and ST. Protein accumulation of up to 0.05% total soluble protein is 
      detected. Subsequently, mucosal and systemic humoral responses are elicited in 
      mice orally dosed with transgenic tobacco leaves. This has suggested that the 
      plant-derived LTB:ST is immunogenic via the oral route. These findings are 
      critical for the development of a plant-based vaccine capable of eliciting 
      broader protection against ETEC and targeting both LTB and ST. Features of this 
      platform in comparison to transplastomic approaches are discussed.
FAU - Rosales-Mendoza, Sergio
AU  - Rosales-Mendoza S
AD  - Laboratorio de Biofarmaceuticos recombinantes, Facultad de Ciencias Quimicas, 
      Universidad Autonoma de San Luis Potosi, Av. Dr. Manuel Nava 6, 78210 San Luis 
      Potosi, SLP, Mexico.
FAU - Soria-Guerra, Ruth E
AU  - Soria-Guerra RE
FAU - Moreno-Fierros, Leticia
AU  - Moreno-Fierros L
FAU - Govea-Alonso, Dania O
AU  - Govea-Alonso DO
FAU - Herrera-Diaz, Areli
AU  - Herrera-Diaz A
FAU - Korban, Schuyler S
AU  - Korban SS
FAU - Alpuche-Solis, Angel G
AU  - Alpuche-Solis AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110212
PL  - Germany
TA  - Plant Cell Rep
JT  - Plant cell reports
JID - 9880970
RN  - 0 (Antigens)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Protein Subunits)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (heat stable toxin (E coli))
SB  - IM
MH  - Administration, Oral
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Formation/immunology
MH  - Antigens/immunology
MH  - Bacterial Toxins/*metabolism
MH  - Base Sequence
MH  - Cell Nucleus/*metabolism
MH  - Enterotoxins/*metabolism
MH  - Escherichia coli/*metabolism
MH  - Escherichia coli Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Plants, Genetically Modified
MH  - Polymerase Chain Reaction
MH  - Protein Subunits/*metabolism
MH  - Recombinant Fusion Proteins/administration & 
      dosage/chemistry/genetics/*immunology
MH  - Nicotiana/*genetics
EDAT- 2011/02/15 06:00
MHDA- 2011/08/25 06:00
CRDT- 2011/02/15 06:00
PHST- 2011/01/03 00:00 [received]
PHST- 2011/01/21 00:00 [accepted]
PHST- 2011/01/21 00:00 [revised]
PHST- 2011/02/15 06:00 [entrez]
PHST- 2011/02/15 06:00 [pubmed]
PHST- 2011/08/25 06:00 [medline]
AID - 10.1007/s00299-011-1023-0 [doi]
PST - ppublish
SO  - Plant Cell Rep. 2011 Jun;30(6):1145-52. doi: 10.1007/s00299-011-1023-0. Epub 2011 
      Feb 12.