PMID- 21318791
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20110214
IS  - 1543-1894 (Print)
IS  - 1543-1894 (Linking)
VI  - 53
DP  - 2001
TI  - Fluorescence In Situ Hybridization (FISH) to Metaphase and Interphase 
      Chromosomes.
PG  - 101-23
LID - 10.1385/1-59259-144-2:101 [doi]
AB  - The unambiguous identification of human chromosomes became possible with the 
      discovery and implementation of G-banding techniques (1). Almost immediately, 
      investigators developed various methods to physically map specific DNA sequences 
      to banded chromosomes. A commonly used early technique involved the hybridization 
      in situ of radioactively labeled probes to heat-denatured human metaphase 
      chromosomes (reviewed in 2). These techniques were efficient, yet costly, 
      time-consuming, and technically difficult. Isotopic hybridization in situ was 
      rapidly superseded by nonisotopic techniques-especially those utilizing 
      fluorescently labeled probes (3-6). This chapter describes basic methodology for 
      the accomplishment of metaphase and interphase fluorescence in situ hybridization 
      (FISH).
FAU - Macoska, J A
AU  - Macoska JA
AD  - Section of Urology, Department of Surgery, and the Program in Cancer Biology, 
      University of Michigan Medical Center, Ann Arbor, MI.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Med
JT  - Methods in molecular medicine
JID - 101123138
EDAT- 2001/01/01 00:00
MHDA- 2001/01/01 00:01
CRDT- 2011/02/15 06:00
PHST- 2011/02/15 06:00 [entrez]
PHST- 2001/01/01 00:00 [pubmed]
PHST- 2001/01/01 00:01 [medline]
AID - 10.1385/1-59259-144-2:101 [doi]
PST - ppublish
SO  - Methods Mol Med. 2001;53:101-23. doi: 10.1385/1-59259-144-2:101.