PMID- 21319369
OWN - NLM
STAT- MEDLINE
DCOM- 20110503
LR  - 20220722
IS  - 1008-9292 (Print)
IS  - 1008-9292 (Linking)
VI  - 40
IP  - 1
DP  - 2011 Jan
TI  - [Determination of four effective components from total flavonoids of Scutellaria 
      barbata by high performance liquid chromatography].
PG  - 23-6
AB  - OBJECTIVE: To establish a HPLC method for simultaneous determination of 4 
      effective components from total flavonoids of Scutellaria barbata (FSB). METHODS: 
      The HPLC method was developed on an Agilent Zorbax C(1)(8) column (4.6 mm x 250 mm, 5 
      mum). The mobile phase was composed of 1% HAc and CH(3)OH:CH(3)CN (80:20) with a 
      linear gradient elution. The flow rate was 1.0 ml/min, and UV detection wave 
      length was set at 280 nm. The column temperature was maintained at 30 degrees C. RESULT: 
      The linear range of 4 effective components (scutellarin, 
      isoscutellarein-8-O-glucuronide, isoscutellarein and luteolin) was 0.14-11.20 mug, 
      0.03-2.40 mug, 0.007-0.560 mug and 0.027-2.160 mug, respectively. The average 
      recovery for 4 effective components was (101.9 +/- 1.4)%, (103.5 +/- 0.6)%, (98.1 +/- 
      2.9)% and (100.5 +/- 2.3)%, respectively. The contents of 4 flavonoids were 
      determined, with scutellarin 7.3%-14.3%, isoscutellarein-8-O-glucuronide 
      2.4%-9.3%, isoscutellarein 0.3%-0.5%, and luteolin 0.2%-0.6%, respectively. 
      CONCLUSION: The method can be used effectively to evaluate the quality of FSB.
FAU - Luan, Lian-jun
AU  - Luan LJ
AD  - Department of Chinese Materia Medica, Zhejiang University, Hangzhou 310058, 
      China.
FAU - Wang, Ye-fei
AU  - Wang YF
FAU - Wu, Yong-jiang
AU  - Wu YJ
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhejiang Da Xue Xue Bao Yi Xue Ban
JT  - Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical 
      sciences
JID - 100927946
RN  - 0 (Flavones)
RN  - 0 (Flavonoids)
RN  - 0 (Glucuronates)
RN  - 16IGP0ML9A (scutellarin)
RN  - 3WAC3UX5SC (isoscutellarein)
RN  - 7V515PI7F6 (Apigenin)
RN  - KUX1ZNC9J2 (Luteolin)
SB  - IM
MH  - Apigenin/analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Flavones/analysis
MH  - Flavonoids/*analysis
MH  - Glucuronates/analysis
MH  - Luteolin/analysis
MH  - Scutellaria/*chemistry
EDAT- 2011/02/15 06:00
MHDA- 2011/05/04 06:00
CRDT- 2011/02/15 06:00
PHST- 2011/02/15 06:00 [entrez]
PHST- 2011/02/15 06:00 [pubmed]
PHST- 2011/05/04 06:00 [medline]
AID - 10.3785/j.issn.1008-9292.2011.01.005 [doi]
PST - ppublish
SO  - Zhejiang Da Xue Xue Bao Yi Xue Ban. 2011 Jan;40(1):23-6. doi: 
      10.3785/j.issn.1008-9292.2011.01.005.