PMID- 21323573
OWN - NLM
STAT- MEDLINE
DCOM- 20110711
LR  - 20110506
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Linking)
VI  - 30
IP  - 5
DP  - 2011 May
TI  - Association of common variants of vascular endothelial growth factor and 
      interleukin-18 genes with allograft survival in renal transplant recipients of 
      North India.
PG  - 309-15
LID - 10.1089/dna.2010.1138 [doi]
AB  - Increased vascular endothelial growth factor (VEGF) production promotes enhanced 
      endothelial permeability, enhanced leukocyte migration into the allograft, 
      thereby leading to a clinically recognized rejection episode. Interleukin-18 
      (IL-18), a potent proinflammatory cytokine, may also be involved in mechanisms of 
      kidney allograft rejection. The present study was, therefore, undertaken to 
      investigate the association of functional polymorphisms in VEGF (2578C>A, 
      1154A>G) and IL-18 (607C>A, 137G>C) genes with risk of allograft rejection in 
      renal transplant recipients of North India. Two hundred renal transplant 
      recipients, 150 matched recipients-donors, and 200 unrelated healthy individuals 
      were genotyped by amplification refractory mutation specific polymerase chain 
      reaction and by polymerase chain reaction-restriction fragment length 
      polymorphism. Variant allele VEGF 1154A>G (p = 0.56; odds ratio [OR] = 1.32) and 
      variant allele (p = 0.004, OR = 1.54) and variant genotype (p = 0.007, OR = 3.26) 
      of IL-18 607C>A, GC of IL-18 137G>C (p = 0.043, OR = 0.63) were significantly 
      different in healthy individuals as compared with the patients with renal 
      transplant. When 114 nonrejectors were compared with 36 rejectors (150 
      recipients) for association with allograft rejection, significant association was 
      observed in heterozygous genotype of VEGF 2578C>A (p = 0.033), VEGF 1154A>G 
      (p = 0.024). In IL-18 137G>C, CC genotype, C allele showed protective association 
      with allograft rejection. Kaplan-Meier analysis indicated a higher mean time for 
      first rejection episode in CA genotype carriers (31 months) as compared with AA 
      (29 months) for VEGF 2578C>A (log p = 0.035). In VEGF, the haplotypes A-A and A-G 
      (2578-1154) were associated with reduced risk and in IL-18 607A-137G, they were 
      associated with high risk for allograft rejection. This observation suggests 
      these polymorphisms are an ideal marker for prediction of pretransplant allograft 
      outcome.
FAU - Mittal, Rama Devi
AU  - Mittal RD
AD  - Department of Urology and Renal Transplantation, Sanjay Gandhi Post Graduate 
      Institute of Medical Sciences, Lucknow, India. ramamittal@gmail.com
FAU - Srivastava, Priyanka
AU  - Srivastava P
FAU - Singh, Vibha
AU  - Singh V
FAU - Jaiswal, Praveen
AU  - Jaiswal P
FAU - Kapoor, Rakesh
AU  - Kapoor R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110216
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (Genetic Markers)
RN  - 0 (Interleukin-18)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Alleles
MH  - Case-Control Studies
MH  - Child
MH  - Female
MH  - Gene Frequency
MH  - Genetic Markers
MH  - Graft Survival/*genetics/*immunology
MH  - Haplotypes
MH  - Humans
MH  - India
MH  - Interleukin-18/*genetics
MH  - Kidney Transplantation/*immunology
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Single Nucleotide
MH  - Risk Factors
MH  - Vascular Endothelial Growth Factor A/*genetics
MH  - Young Adult
EDAT- 2011/02/18 06:00
MHDA- 2011/07/12 06:00
CRDT- 2011/02/18 06:00
PHST- 2011/02/18 06:00 [entrez]
PHST- 2011/02/18 06:00 [pubmed]
PHST- 2011/07/12 06:00 [medline]
AID - 10.1089/dna.2010.1138 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2011 May;30(5):309-15. doi: 10.1089/dna.2010.1138. Epub 2011 Feb 
      16.