PMID- 21323721
OWN - NLM
STAT- MEDLINE
DCOM- 20110705
LR  - 20220318
IS  - 1600-0560 (Electronic)
IS  - 0303-6987 (Linking)
VI  - 38
IP  - 4
DP  - 2011 Apr
TI  - Fluorescence in situ hybridization as an ancillary method for the distinction of 
      desmoplastic melanomas from sclerosing melanocytic nevi.
PG  - 329-34
LID - 10.1111/j.1600-0560.2010.01666.x [doi]
AB  - The histopathologic distinction of desmoplastic melanomas from sclerosing 
      (desmoplastic) melanocytic nevi can be difficult, especially when evaluating a 
      partial or superficial biopsy. In the study reported herein, we applied and 
      explored the use of a novel ancillary method, a four-probe fluorescence in situ 
      hybridization (FISH) assay targeting RREB1, MYB, Cep6 and CCND1, to this 
      diagnostic problem. Fifteen sclerosing melanocytic nevi, including desmoplastic 
      Spitz nevi, conventional nevi with prominent stromal sclerosis and sclerotic blue 
      nevi, as well as 15 examples of desmoplastic melanoma, were examined. None of the 
      sclerosing melanocytic nevi showed a level of chromosomal aberrations that met 
      FISH criteria for melanoma. Seven of the 15 desmoplastic melanomas were 
      'positive' (had documented chromosomal aberrations) by FISH. Thus, a positive 
      FISH test strongly supports the diagnosis of melanoma in this context. However, 
      in this setting a negative FISH test is of limited diagnostic value. Our findings 
      suggest that prior reports about the high sensitivity of the FISH test for 
      melanoma diagnosis need to be adjusted according to melanoma subtype.
CI  - Copyright (c) 2011 John Wiley & Sons A/S.
FAU - Gerami, Pedram
AU  - Gerami P
AD  - Department of Dermatology, Northwestern University, Feinberg School of Medicine, 
      Chicago, USA.
FAU - Beilfuss, Beth
AU  - Beilfuss B
FAU - Haghighat, Zahra
AU  - Haghighat Z
FAU - Fang, Yuaquin
AU  - Fang Y
FAU - Jhanwar, Suresh
AU  - Jhanwar S
FAU - Busam, Klaus J
AU  - Busam KJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cutan Pathol
JT  - Journal of cutaneous pathology
JID - 0425124
SB  - IM
CIN - J Cutan Pathol. 2011 Apr;38(4):327-8. PMID: 21323720
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Melanoma/*diagnosis/genetics
MH  - Middle Aged
MH  - Nevus, Pigmented/*diagnosis/genetics
MH  - Skin Neoplasms/*diagnosis/genetics
EDAT- 2011/02/18 06:00
MHDA- 2011/07/06 06:00
CRDT- 2011/02/18 06:00
PHST- 2011/02/18 06:00 [entrez]
PHST- 2011/02/18 06:00 [pubmed]
PHST- 2011/07/06 06:00 [medline]
AID - 10.1111/j.1600-0560.2010.01666.x [doi]
PST - ppublish
SO  - J Cutan Pathol. 2011 Apr;38(4):329-34. doi: 10.1111/j.1600-0560.2010.01666.x.