PMID- 21323960
OWN - NLM
STAT- MEDLINE
DCOM- 20110712
LR  - 20110302
IS  - 1365-2559 (Electronic)
IS  - 0309-0167 (Linking)
VI  - 58
IP  - 3
DP  - 2011 Feb
TI  - Immunohistochemical analysis of p53, APE1, hMSH2 and ERCC1 proteins in actinic 
      cheilitis and lip squamous cell carcinoma.
PG  - 352-60
LID - 10.1111/j.1365-2559.2011.03756.x [doi]
AB  - AIMS: This study has compared the tissue expression of the p53 tumour suppressor 
      protein and DNA repair proteins APE1, hMSH2 and ERCC1 in normal, dysplastic and 
      malignant lip epithelium. METHODS AND RESULTS: Morphological analysis and 
      immunohistochemistry were performed on archived specimens of normal lip mucosa 
      (n=15), actinic cheilitis (AC) (n=30), and lip squamous cell carcinoma (LSCC) 
      (n=27). AC samples were classified morphologically according to the severity of 
      epithelial dysplasia and risk of malignant transformation. LSCC samples were 
      morphologically staged according to WHO and invasive front grading (IFG) 
      criteria. Differences between groups and morphological stages were determined by 
      bivariate statistical analysis. Progressive increases in the percentage of 
      epithelial cells expressing p53 and APE1 were associated with increases in 
      morphological malignancy from normal lip mucosa to LSCC. There was also a 
      significant reduction in epithelial cells expressing hMSH2 and ERCC1 proteins in 
      the AC and LSCC groups. A higher percentage of malignant cells expressing APE1 
      was found in samples with an aggressive morphological IFG grade. CONCLUSIONS: Our 
      data showed that epithelial cells from premalignant to malignant lip disease 
      exhibited changes in the expression of p53, APE1, hMSH2 and ERCC1 proteins; these 
      molecular change might contribute to lip carcinogenesis.
CI  - (c) 2011 Blackwell Publishing Limited.
FAU - Souza, Ludmilla R
AU  - Souza LR
AD  - Health Science Programme, State University of Montes Claros, Montes Claros, MG, 
      Brazil.
FAU - Fonseca-Silva, Thiago
AU  - Fonseca-Silva T
FAU - Pereira, Camila S
AU  - Pereira CS
FAU - Santos, Erivelton P
AU  - Santos EP
FAU - Lima, Lucianne C
AU  - Lima LC
FAU - Carvalho, Heloisa A
AU  - Carvalho HA
FAU - Gomez, Ricardo S
AU  - Gomez RS
FAU - Guimaraes, Andre L S
AU  - Guimaraes AL
FAU - De Paula, Alfredo M B
AU  - De Paula AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110216
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 3.1.- (ERCC1 protein, human)
RN  - EC 3.1.- (Endonucleases)
RN  - EC 3.6.1.3 (MSH2 protein, human)
RN  - EC 3.6.1.3 (MutS Homolog 2 Protein)
RN  - EC 4.2.99.18 (APEX1 protein, human)
RN  - EC 4.2.99.18 (DNA-(Apurinic or Apyrimidinic Site) Lyase)
RN  - Actinic cheilitis
SB  - IM
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Cell Differentiation
MH  - Cheilitis/metabolism/pathology
MH  - DNA-(Apurinic or Apyrimidinic Site) Lyase/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Endonucleases/*metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Lip Neoplasms/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - MutS Homolog 2 Protein/*metabolism
MH  - Retrospective Studies
MH  - Tumor Suppressor Protein p53/*metabolism
EDAT- 2011/02/18 06:00
MHDA- 2011/07/13 06:00
CRDT- 2011/02/18 06:00
PHST- 2011/02/18 06:00 [entrez]
PHST- 2011/02/18 06:00 [pubmed]
PHST- 2011/07/13 06:00 [medline]
AID - 10.1111/j.1365-2559.2011.03756.x [doi]
PST - ppublish
SO  - Histopathology. 2011 Feb;58(3):352-60. doi: 10.1111/j.1365-2559.2011.03756.x. 
      Epub 2011 Feb 16.