PMID- 21325295
OWN - NLM
STAT- MEDLINE
DCOM- 20110815
LR  - 20210103
IS  - 1557-3265 (Electronic)
IS  - 1078-0432 (Linking)
VI  - 17
IP  - 4
DP  - 2011 Feb 15
TI  - Natural killer cells are scarce in colorectal carcinoma tissue despite high 
      levels of chemokines and cytokines.
PG  - 678-89
LID - 10.1158/1078-0432.CCR-10-2173 [doi]
AB  - PURPOSE: Tumor infiltrating T lymphocytes in colorectal cancer (CRC) have 
      prognostic impact, but the role of natural killer (NK) cells in CRC tissue is 
      unclear. The contribution of intratumoral cytokines and chemokines in shaping the 
      composition of the inflammatory lymphocytic infiltrate is also unclear. 
      EXPERIMENTAL DESIGN: In this study, localization and densities of NK and T cells 
      within primary CRC, CRC liver metastases, adenomas, and normal tissues were 
      analyzed on whole tissue sections from 112 patients. In a subset of these 
      patients, the most important 50 cytokines and chemokines were quantified in 
      paired serum, primary CRC and adjacent mucosa samples and in CRC liver metastases 
      and correlated with NK and T-cell infiltration, respectively. RESULTS: The 
      various compartments displayed characteristic differences like significantly 
      higher chemokine concentrations in CRC tissue. Most importantly, despite high 
      local chemokine levels, NK cells were generally scarce within CRC tumor tissues, 
      independent of human leukocyte antigen (HLA) class I expression. Adjacent normal 
      mucosa contained normal levels of NK cells. In contrast, corresponding T-cell 
      numbers varied substantially and were positively correlated with higher chemokine 
      levels. CONCLUSIONS: Our findings indicate a distinct regulation of NK cells 
      versus T cells in the CRC tumor microenvironment. NK-cell migration into CRC 
      tumor tissue is obviously impaired early during tumor development by mechanisms 
      that do not affect T-cell infiltration.
CI  - (c)2011 AACR.
FAU - Halama, Niels
AU  - Halama N
AD  - Medical Oncology, National Center for Tumor Diseases, University Heidelberg, 
      Germany.
FAU - Braun, Monika
AU  - Braun M
FAU - Kahlert, Christoph
AU  - Kahlert C
FAU - Spille, Anna
AU  - Spille A
FAU - Quack, Christian
AU  - Quack C
FAU - Rahbari, Nuh
AU  - Rahbari N
FAU - Koch, Moritz
AU  - Koch M
FAU - Weitz, Jurgen
AU  - Weitz J
FAU - Kloor, Matthias
AU  - Kloor M
FAU - Zoernig, Inka
AU  - Zoernig I
FAU - Schirmacher, Peter
AU  - Schirmacher P
FAU - Brand, Karsten
AU  - Brand K
FAU - Grabe, Niels
AU  - Grabe N
FAU - Falk, Christine S
AU  - Falk CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for 
      Cancer Research
JID - 9502500
RN  - 0 (CD56 Antigen)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (NCAM1 protein, human)
RN  - 0 (NCR1 protein, human)
RN  - 0 (Natural Cytotoxicity Triggering Receptor 1)
RN  - 0 (Selectins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - CD56 Antigen/metabolism
MH  - Cell Adhesion Molecules/metabolism
MH  - Cell Movement
MH  - Chemokines/*metabolism
MH  - Colorectal Neoplasms/metabolism/*pathology
MH  - Cytokines/*metabolism
MH  - Female
MH  - Humans
MH  - Intestinal Mucosa/pathology
MH  - Killer Cells, Natural/*metabolism
MH  - Liver/pathology
MH  - Liver Neoplasms/metabolism/pathology/secondary
MH  - Male
MH  - Middle Aged
MH  - Natural Cytotoxicity Triggering Receptor 1/metabolism
MH  - Selectins/metabolism
MH  - T-Lymphocytes/metabolism
EDAT- 2011/02/18 06:00
MHDA- 2011/08/16 06:00
CRDT- 2011/02/18 06:00
PHST- 2011/02/18 06:00 [entrez]
PHST- 2011/02/18 06:00 [pubmed]
PHST- 2011/08/16 06:00 [medline]
AID - 17/4/678 [pii]
AID - 10.1158/1078-0432.CCR-10-2173 [doi]
PST - ppublish
SO  - Clin Cancer Res. 2011 Feb 15;17(4):678-89. doi: 10.1158/1078-0432.CCR-10-2173.