PMID- 21325485
OWN - NLM
STAT- MEDLINE
DCOM- 20110725
LR  - 20220311
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 18
IP  - 4
DP  - 2011 Apr
TI  - Development and evaluation of a latex agglutination test for the serodiagnosis of 
      paracoccidioidomycosis.
PG  - 604-8
LID - 10.1128/CVI.00130-10 [doi]
AB  - Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin 
      America. It is caused by the dimorphic fungus Paracoccidioides brasiliensis. The 
      immunodiffusion (ID) test is one of the most widely used techniques for PCM 
      serologic diagnosis due to the simplicity and low costs of its execution. 
      However, it requires trained and qualified people to execute it. The purpose of 
      this study was to evaluate a latex particle agglutination (LA) test for the 
      detection of anti-P. brasiliensis antibodies by using pooled crude exoantigens 
      from the fungus. Fifty-one serum samples obtained from patients with PCM were 
      tested. Positivity was observed in 84% (43/51) of these patients, and the 
      agglutination patterns varied from small clumps with a cloudy background to large 
      clumps with a clear background. The antibody titer reactivity ranged from 1:2 to 
      1:64. Cross-reactivity was observed in sera from patients with aspergillosis, 
      histoplasmosis, and nonfungal disease. Serum samples obtained from healthy donors 
      were not reactive. The sensitivity and specificity of the LA test were 84% and 
      81%, respectively. When comparing the LA test with the double-immunodiffusion 
      test, we found an agreement of 92%. Further work is needed to improve the 
      performance of the LA assay before it can be proposed as a reliable diagnostic 
      tool, mainly in laboratories with little infrastructure.
FAU - Silveira-Gomes, Fabiola
AU  - Silveira-Gomes F
AD  - Federal University of Para, Science Biologic Institute, Belem, Para, Brazil.
FAU - Sarmento, Dayse Nogueira
AU  - Sarmento DN
FAU - Pinto, Thifany Mendes
AU  - Pinto TM
FAU - Pimentel, Rosiane Ferreira
AU  - Pimentel RF
FAU - Nepomuceno, Livia Barreto
AU  - Nepomuceno LB
FAU - Espirito Santo, Elaine Patricia T
AU  - Espirito Santo EP
FAU - Mesquita-da-Costa, Maurimelia
AU  - Mesquita-da-Costa M
FAU - Camargo, Zoilo Pires
AU  - Camargo ZP
FAU - Marques-da-Silva, Silvia Helena
AU  - Marques-da-Silva SH
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110216
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antibodies, Fungal/*blood
MH  - Antigens, Fungal
MH  - Clinical Laboratory Techniques/*methods
MH  - Female
MH  - Humans
MH  - Latex Fixation Tests/methods
MH  - Latin America
MH  - Male
MH  - Middle Aged
MH  - Paracoccidioides/immunology
MH  - Paracoccidioidomycosis/*diagnosis
MH  - Sensitivity and Specificity
MH  - Young Adult
PMC - PMC3122578
EDAT- 2011/02/18 06:00
MHDA- 2011/07/26 06:00
PMCR- 2011/10/01
CRDT- 2011/02/18 06:00
PHST- 2011/02/18 06:00 [entrez]
PHST- 2011/02/18 06:00 [pubmed]
PHST- 2011/07/26 06:00 [medline]
PHST- 2011/10/01 00:00 [pmc-release]
AID - CVI.00130-10 [pii]
AID - 0130-10 [pii]
AID - 10.1128/CVI.00130-10 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2011 Apr;18(4):604-8. doi: 10.1128/CVI.00130-10. Epub 2011 
      Feb 16.