PMID- 21325559
OWN - NLM
STAT- MEDLINE
DCOM- 20110718
LR  - 20240320
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 49
IP  - 4
DP  - 2011 Apr
TI  - Preferential deletion events in the direct repeat locus of Mycobacterium 
      tuberculosis.
PG  - 1318-22
LID - 10.1128/JCM.01848-10 [doi]
AB  - The "Harlingen" IS6110 restriction fragment length polymorphism (RFLP) cluster 
      has linked over 100 tuberculosis cases in The Netherlands since 1993. Four 
      Mycobacterium tuberculosis isolates that were epidemiologically linked to this 
      cluster had different spoligotype patterns, as well as slightly divergent IS6110 
      profiles, compared to the majority of the isolates. Sequencing of the direct 
      repeat (DR) locus revealed sequence polymorphisms at the putative deletion sites. 
      These deletion footprints provided evidence for independent deletions of the 
      central region of the DR locus in three isolates, while the different genotype of 
      the fourth isolate was explained by transmission. Our finding suggests that 
      convergent deletions in the DR locus occur frequently. However, deletion 
      footprints are not suitable to detect convergent deletions in the DR because they 
      seem to be exceptional. Deletion footprints in the DR were not described 
      previously, and we did not observe them in any public M. tuberculosis complex 
      sequences. We conclude that preferential deletions in the DR loci of closely 
      related strains are usually an unnoted event that interferes with clustering of 
      closely related strains.
FAU - Schurch, Anita C
AU  - Schurch AC
AD  - Tuberculosis Reference Laboratory, National Institute for Public Health and 
      Environment (RIVM), Centre for Infectious Disease Control (CIbpSH-D2), Laboratory 
      for Infectious Disease and Perinatal Screening, P.O. Box 1, 3720 BA Bilthoven, 
      the Netherlands.
FAU - Kremer, Kristin
AU  - Kremer K
FAU - Kiers, Albert
AU  - Kiers A
FAU - Boeree, Martin J
AU  - Boeree MJ
FAU - Siezen, Roland J
AU  - Siezen RJ
FAU - van Soolingen, Dick
AU  - van Soolingen D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110216
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Bacterial Typing Techniques
MH  - DNA Transposable Elements
MH  - DNA, Bacterial/*genetics
MH  - Genotype
MH  - Humans
MH  - Molecular Epidemiology
MH  - Molecular Typing
MH  - Mycobacterium tuberculosis/*genetics
MH  - Netherlands/epidemiology
MH  - Polymorphism, Genetic
MH  - Polymorphism, Restriction Fragment Length
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Sequence Analysis, DNA
MH  - *Sequence Deletion
MH  - Tuberculosis/*epidemiology/microbiology
PMC - PMC3122804
EDAT- 2011/02/18 06:00
MHDA- 2011/07/19 06:00
PMCR- 2011/10/01
CRDT- 2011/02/18 06:00
PHST- 2011/02/18 06:00 [entrez]
PHST- 2011/02/18 06:00 [pubmed]
PHST- 2011/07/19 06:00 [medline]
PHST- 2011/10/01 00:00 [pmc-release]
AID - JCM.01848-10 [pii]
AID - 1848-10 [pii]
AID - 10.1128/JCM.01848-10 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2011 Apr;49(4):1318-22. doi: 10.1128/JCM.01848-10. Epub 2011 
      Feb 16.