PMID- 21325632
OWN - NLM
STAT- MEDLINE
DCOM- 20110816
LR  - 20211020
IS  - 1939-4586 (Electronic)
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 22
IP  - 8
DP  - 2011 Apr 15
TI  - MYADM regulates Rac1 targeting to ordered membranes required for cell spreading 
      and migration.
PG  - 1252-62
LID - 10.1091/mbc.E10-11-0910 [doi]
AB  - Membrane organization into condensed domains or rafts provides molecular 
      platforms for selective recruitment of proteins. Cell migration is a general 
      process that requires spatiotemporal targeting of Rac1 to membrane rafts. The 
      protein machinery responsible for making rafts competent to recruit Rac1 remains 
      elusive. Some members of the MAL family of proteins are involved in specialized 
      processes dependent on this type of membrane. Because condensed membrane domains 
      are a general feature of the plasma membrane of all mammalian cells, we 
      hypothesized that MAL family members with ubiquitous expression and plasma 
      membrane distribution could be involved in the organization of membranes for cell 
      migration. We show that myeloid-associated differentiation marker (MYADM), a 
      protein with unique features within the MAL family, colocalizes with Rac1 in 
      membrane protrusions at the cell surface and distributes in condensed membranes. 
      MYADM knockdown (KD) cells had altered membrane condensation and showed deficient 
      incorporation of Rac1 to membrane raft fractions and, similar to Rac1 KD cells, 
      exhibited reduced cell spreading and migration. Results of rescue-of-function 
      experiments by expression of MYADM or active Rac1L61 in cells knocked down for 
      Rac1 or MYADM, respectively, are consistent with the idea that MYADM and Rac1 act 
      on parallel pathways that lead to similar functional outcomes.
FAU - Aranda, Juan F
AU  - Aranda JF
AD  - Centro de Biologia Molecular Severo Ochoa, Cantoblanco, 28049 Madrid, Spain.
FAU - Reglero-Real, Natalia
AU  - Reglero-Real N
FAU - Kremer, Leonor
AU  - Kremer L
FAU - Marcos-Ramiro, Beatriz
AU  - Marcos-Ramiro B
FAU - Ruiz-Saenz, Ana
AU  - Ruiz-Saenz A
FAU - Calvo, Maria
AU  - Calvo M
FAU - Enrich, Carlos
AU  - Enrich C
FAU - Correas, Isabel
AU  - Correas I
FAU - Millan, Jaime
AU  - Millan J
FAU - Alonso, Miguel A
AU  - Alonso MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110216
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Antigens, Differentiation)
RN  - 0 (MYADM protein, human)
RN  - 0 (Myelin and Lymphocyte-Associated Proteolipid Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - EC 3.6.5.2 (rac1 GTP-Binding Protein)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation/genetics/*metabolism
MH  - Cell Adhesion
MH  - Cell Line, Tumor
MH  - Cell Membrane/metabolism
MH  - *Cell Movement
MH  - Dogs
MH  - Electroporation
MH  - Female
MH  - Gene Expression
MH  - Gene Silencing
MH  - Haplorhini
MH  - Humans
MH  - Male
MH  - Membrane Microdomains/*metabolism
MH  - Myelin and Lymphocyte-Associated Proteolipid Proteins
MH  - Phylogeny
MH  - Proteins/genetics/*metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Transfection
MH  - rac1 GTP-Binding Protein/genetics/*metabolism
PMC - PMC3078064
EDAT- 2011/02/18 06:00
MHDA- 2011/08/17 06:00
PMCR- 2011/06/30
CRDT- 2011/02/18 06:00
PHST- 2011/02/18 06:00 [entrez]
PHST- 2011/02/18 06:00 [pubmed]
PHST- 2011/08/17 06:00 [medline]
PHST- 2011/06/30 00:00 [pmc-release]
AID - mbc.E10-11-0910 [pii]
AID - E10-11-0910 [pii]
AID - 10.1091/mbc.E10-11-0910 [doi]
PST - ppublish
SO  - Mol Biol Cell. 2011 Apr 15;22(8):1252-62. doi: 10.1091/mbc.E10-11-0910. Epub 2011 
      Feb 16.