PMID- 21327048
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20170823
LR  - 20211020
IS  - 1938-1964 (Electronic)
IS  - 1938-1956 (Print)
IS  - 1938-1964 (Linking)
VI  - 1
IP  - 2
DP  - 2010 Oct
TI  - Cells from a vanished twin as a source of microchimerism 40 years later.
PG  - 56-60
LID - 10.4161/chim.1.2.14294 [doi]
AB  - We report the case of a 40-year-old man diagnosed with a scleroderma-like 
      disease. Clinical similarities with graft versus host disease prompted initial 
      testing for chimerism employing fluorescence in situ hybridization (FISH). Female 
      cells were observed within peripheral blood mononuclear cells from the 
      patient.Because maternal cells have been detected in healthy immunologically 
      competent adults and patients with autoimmune conditions, we hypothesized that 
      these cells were of maternal origin. Contrary to our expectations, HLA-specific 
      quantitative PCR (QPCR) ruled out maternal microchimerism. However, HLA-specific 
      QPCR testing was positive for the paternal HLA haplotype that the patient did not 
      inherit. We reasoned that the most likely origin of chimerism with non-inherited 
      paternal HLA alleles was from an unrecognized "vanished" twin. The patient had 
      never received a blood transfusion.This report suggests that cells from a 
      vanished twin are a possible source of chimerism. The frequency of chimerism from 
      this source is not yet known and whether the scleroderma-like disease observed in 
      the patient is anecdotal or implies a potential association with autoimmune 
      disease remains to be elucidated.
FAU - de Bellefon, Laurent Meric
AU  - de Bellefon LM
AD  - Institute for Medical Immunology; Hopital Erasme; Universite libre de Bruxelles; 
      Belgium.
FAU - Heiman, Pierre
AU  - Heiman P
FAU - Kanaan, Sami B
AU  - Kanaan SB
FAU - Azzouz, Doua F
AU  - Azzouz DF
FAU - Rak, Justyna M
AU  - Rak JM
FAU - Martin, Marielle
AU  - Martin M
FAU - Roudier, Jean
AU  - Roudier J
FAU - Roufosse, Florence
AU  - Roufosse F
FAU - Lambert, Nathalie C
AU  - Lambert NC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Chimerism
JT  - Chimerism
JID - 101540510
PMC - PMC3023624
EDAT- 2011/02/18 06:00
MHDA- 2011/02/18 06:01
PMCR- 2010/10/01
CRDT- 2011/02/18 06:00
PHST- 2010/11/12 00:00 [received]
PHST- 2010/11/22 00:00 [revised]
PHST- 2010/11/29 00:00 [accepted]
PHST- 2011/02/18 06:00 [entrez]
PHST- 2011/02/18 06:00 [pubmed]
PHST- 2011/02/18 06:01 [medline]
PHST- 2010/10/01 00:00 [pmc-release]
AID - 1938-1956-1-2-3 [pii]
AID - 10.4161/chim.1.2.14294 [doi]
PST - ppublish
SO  - Chimerism. 2010 Oct;1(2):56-60. doi: 10.4161/chim.1.2.14294.