PMID- 21327437
OWN - NLM
STAT- MEDLINE
DCOM- 20121207
LR  - 20211020
IS  - 1437-160X (Electronic)
IS  - 0172-8172 (Linking)
VI  - 32
IP  - 6
DP  - 2012 Jun
TI  - Effects of pulsed electromagnetic fields on the mRNA expression of CAII and RANK 
      in ovariectomized rats.
PG  - 1527-32
LID - 10.1007/s00296-010-1740-7 [doi]
AB  - The present study was designed to determine the effects of pulsed electromagnetic 
      fields (PEMFs) on the mRNA expression of the carbonic anhydrase II (CAII) and 
      receptor activator of NF-kappaB (RANK) in ovariectomized rats. A total of 48 SD rats 
      were randomly divided into four groups [Sham, OVX, PEMFs, and E(2) (premarin)], 
      12 rats in each group. Rats in the Sham group received sham ovariectomy, while 
      rats in OVX, PEMFs, and E(2) groups received ovariectomy. Twelve weeks following 
      the surgery, rats (whole body) in the PEMFs group were exposed to PEMFs for 
      30 days with 3.8 mT, 8 Hz, and 40 min per day; rats in the E(2) group were 
      administered premarin (0.0625 mg/kg/d; intragastric administration 1-2 ml/100 g). 
      Rats in the Sham and OVX groups housed in the same conditions. At the end of 
      intervention, the level of serum estradiol of rats was measured. The gene 
      expression of CAII and RANK in the left ilium of rats was determined with 
      real-time fluorescent-nested quantitative polymerase chain reaction. Compared 
      with the Sham group, the level of serum estradiol in the ovariectomized group was 
      significantly decreased (P < 0.05); compared with the OVX group, CAIImRNA 
      expression was significantly decreased in the PEMFs group and E group (P < 0.05, 
      0.01, respectively). Compared with the E group, RANKmRNA expression was 
      significantly higher in the PEMFs group (P < 0.05); although RANKmRNA expression 
      decreased in PEMFs group, no statistically significant difference was found 
      between PEMF group and OVX group (P = 0.82). These data suggest that PEMFs could 
      regulate the expression of CAIImRNA in ovariectomized rats.
FAU - Chen, Jian
AU  - Chen J
AD  - Department of Rehabilitation, Zhong Shang Hospital, Xiamen University, Xiamen, 
      361004, China.
FAU - Huang, Li-Qun
AU  - Huang LQ
FAU - Xia, Qing-Jie
AU  - Xia QJ
FAU - He, Cheng-Qi
AU  - He CQ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110215
PL  - Germany
TA  - Rheumatol Int
JT  - Rheumatology international
JID - 8206885
RN  - 0 (Estrogens)
RN  - 0 (Estrogens, Conjugated (USP))
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor Activator of Nuclear Factor-kappa B)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Animals
MH  - Carbonic Anhydrase II/*genetics
MH  - *Electromagnetic Fields
MH  - Estradiol/administration & dosage/blood
MH  - Estrogen Replacement Therapy
MH  - Estrogens/administration & dosage/blood
MH  - Estrogens, Conjugated (USP)/administration & dosage/blood
MH  - Female
MH  - Gene Expression Regulation, Enzymologic
MH  - Ilium/drug effects/*enzymology
MH  - *Ovariectomy
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptor Activator of Nuclear Factor-kappa B/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
EDAT- 2011/02/18 06:00
MHDA- 2012/12/12 06:00
CRDT- 2011/02/18 06:00
PHST- 2010/08/06 00:00 [received]
PHST- 2010/12/30 00:00 [accepted]
PHST- 2011/02/18 06:00 [entrez]
PHST- 2011/02/18 06:00 [pubmed]
PHST- 2012/12/12 06:00 [medline]
AID - 10.1007/s00296-010-1740-7 [doi]
PST - ppublish
SO  - Rheumatol Int. 2012 Jun;32(6):1527-32. doi: 10.1007/s00296-010-1740-7. Epub 2011 
      Feb 15.