PMID- 21328977
OWN - NLM
STAT- MEDLINE
DCOM- 20140123
LR  - 20110217
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 26
IP  - 4
DP  - 2010 Nov
TI  - [Study of the effects of Auricularia auricular polysaccharide on local 
      ischemia/reperfusion injury in rat].
PG  - 423-6
AB  - OBJECTIVE: To investigate the influence of Auricularia Auricular polysaccharide 
      (APP) on acute cerebral injury induced by ischemia/reperfusion in rats and its 
      underlying mechanism. METHODS: Adult male SD rats were intragastrically 
      pretreated with AAP at a low (50 mg/kg) or high (100 mg/kg) dose once a day for 
      20 days before operation. Rats intraperitoneally injected with ginkgo biloba 
      extract (EGb671) were taken as positive control. Focal ischemia was achieved by 
      middle cerebral artery occlusion (MCAO) on the right side for 60 min. After 24 
      hrs of reperfusion, the nerve function defects were recorded by Longa's score and 
      the brain infarct sizes were measured by 2,3,5-Triphenyl-tetrazolium-chlor (TTC) 
      staining. Apoptotic neurons were detected by terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) staining after 48 h of 
      reperfusion. The levels of oxidative stress was determined via the 
      mitochondria-generated reactive oxygen species (ROS). RESULTS: AAP treatment 
      decreased Longa's score, brain infarct size, apoptotic neurons and 
      mitochondria-generated ROS in a dose-dependent manner. AAP at 100 mg/kg gave a 
      better performance compared with EGb671 on all parameters examined. CONCLUSION: 
      AAP treatment protected rat brain from focal ischemia/reperfusion injury by its 
      anti-oxidative effect and worked better than EGb671.
FAU - Ye, Ting-Mei
AU  - Ye TM
AD  - Department of Biology, College of Chemistry and Life Sciences, Lishui University, 
      Lishui 323000, China.
FAU - Sun, Li-Na
AU  - Sun LN
FAU - Su, Fang
AU  - Su F
FAU - Shen, Jia
AU  - Shen J
FAU - Wang, Hui-Ping
AU  - Wang HP
FAU - Ye, Zhi-Guo
AU  - Ye ZG
FAU - Xia, Qiang
AU  - Xia Q
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (Polysaccharides)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Basidiomycota/*chemistry
MH  - Brain Ischemia/*metabolism/pathology
MH  - Infarction, Middle Cerebral Artery/metabolism/pathology
MH  - Male
MH  - Oxidative Stress/drug effects
MH  - Polysaccharides/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
MH  - Reperfusion Injury/*metabolism/pathology
EDAT- 2011/02/19 06:00
MHDA- 2014/01/24 06:00
CRDT- 2011/02/19 06:00
PHST- 2011/02/19 06:00 [entrez]
PHST- 2011/02/19 06:00 [pubmed]
PHST- 2014/01/24 06:00 [medline]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2010 Nov;26(4):423-6.