PMID- 2132918
OWN - NLM
STAT- MEDLINE
DCOM- 19910228
LR  - 20190903
IS  - 0367-8377 (Print)
IS  - 0367-8377 (Linking)
VI  - 36
IP  - 2
DP  - 1990 Aug
TI  - Heat stable proteinase from Thermomonospora fusca. Characterization as a serine 
      proteinase.
PG  - 201-7
AB  - An extracellular proteinase secreted by the thermophilic bacteria Thermomonospora 
      fusca YX (YX-proteinase) is a serine proteinase as shown by its inactivation by 
      the site specific reagents, phenylmethanesulfonyl fluoride, dansyl fluoride, and 
      carbobenzoxy-L-phenylalanine chloromethyl ketone. This conclusion is further 
      supported by the effect of various proteinase inhibitors on its activity. The 
      activity of the proteinase toward small synthetic ester substrates shows that the 
      enzyme has a primary specificity for the aromatic and hydrophobic amino acids. 
      The amino acid composition and NH2-terminal sequence, as well as its size, 
      suggest that the enzyme is related to the chymotrypsin-like microbial proteinase, 
      alpha-lytic protease from Myxobacter 495 and protease A and B from Streptomyces 
      griseus.
FAU - Kristjansson, M M
AU  - Kristjansson MM
AD  - Institute of Food Science, Cornell University, Ithaca, NY.
FAU - Kinsella, J E
AU  - Kinsella JE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Int J Pept Protein Res
JT  - International journal of peptide and protein research
JID - 0330420
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
EIN - Int J Pept Protein Res 1991 Sep;38(3):287
MH  - Amino Acid Sequence
MH  - Bacteria/*enzymology
MH  - Enzyme Stability
MH  - *Hot Temperature
MH  - Molecular Sequence Data
MH  - Serine Endopeptidases/chemistry/*isolation & purification/metabolism
MH  - Species Specificity
MH  - Substrate Specificity
EDAT- 1990/08/01 00:00
MHDA- 1990/08/01 00:01
CRDT- 1990/08/01 00:00
PHST- 1990/08/01 00:00 [pubmed]
PHST- 1990/08/01 00:01 [medline]
PHST- 1990/08/01 00:00 [entrez]
AID - 10.1111/j.1399-3011.1990.tb00968.x [doi]
PST - ppublish
SO  - Int J Pept Protein Res. 1990 Aug;36(2):201-7. doi: 
      10.1111/j.1399-3011.1990.tb00968.x.