PMID- 21329180
OWN - NLM
STAT- MEDLINE
DCOM- 20110317
LR  - 20110218
IS  - 0126-8635 (Print)
IS  - 0126-8635 (Linking)
VI  - 32
IP  - 2
DP  - 2010 Dec
TI  - Observation of dendritic cell morphology under light, phase-contrast or confocal 
      laser scanning microscopy.
PG  - 97-102
AB  - Dendritic cells (DCs) are professional antigen presenting cells of the immune 
      system. They can be generated in vitro from peripheral blood monocytes 
      supplemented with GM-CSF, IL-4 and TNF alpha. During induction, DCs will increase 
      in size and acquire multiple cytoplasmic projections when compared to their 
      precursor cells such as monocytes or haematopoietic stem cells which are usually 
      round or spherical. Morphology of DCs can be visualized by conventional light 
      microscopy after staining or phase-contrast inverted microscopy or confocal laser 
      scanning microscopy. In this report, we described the morphological appearances 
      of DCs captured using the above-mentioned techniques. We found that confocal 
      laser scanning microscopy yielded DCs images with greater details but the 
      operating cost for such a technique is high. On the other hand, the images 
      obtained through light microscopy after appropriate staining or phase contrast 
      microscopy were acceptable for identification purpose. Besides, these equipments 
      are readily available in most laboratories and the cost of operation is 
      affordable. Nevertheless, morphological identification is just one of the methods 
      to characterise DCs. Other methods such as phenotypic expression markers and 
      mixed leukocyte reactions are additional tools used in the characterisation of 
      DCs.
FAU - Tan, Yuen-Fen
AU  - Tan YF
AD  - Cell Banking Unit, PPUKM-MAKNA Cancer Centre, Kuala Lumpur, Malaysia.
FAU - Leong, Chooi-Fun
AU  - Leong CF
FAU - Cheong, Soon-Keng
AU  - Cheong SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Malaysia
TA  - Malays J Pathol
JT  - The Malaysian journal of pathology
JID - 8101177
SB  - IM
MH  - Dendritic Cells/*cytology
MH  - Humans
MH  - *Microscopy, Confocal
MH  - *Microscopy, Phase-Contrast
EDAT- 2011/02/19 06:00
MHDA- 2011/03/18 06:00
CRDT- 2011/02/19 06:00
PHST- 2011/02/19 06:00 [entrez]
PHST- 2011/02/19 06:00 [pubmed]
PHST- 2011/03/18 06:00 [medline]
PST - ppublish
SO  - Malays J Pathol. 2010 Dec;32(2):97-102.