PMID- 21329613
OWN - NLM
STAT- MEDLINE
DCOM- 20110623
LR  - 20191112
IS  - 0722-5091 (Print)
IS  - 0722-5091 (Linking)
VI  - 30
IP  - 2
DP  - 2011 Mar-Apr
TI  - FISH-based detection of 1p 19q codeletion in oligodendroglial tumors: procedures 
      and protocols for neuropathological practice - a publication under the auspices 
      of the Research Committee of the European Confederation of Neuropathological 
      Societies (Euro-CNS).
PG  - 47-55
AB  - The codeletion of chromosomal arms 1p 19q is a characteristic and early genetic 
      event in oligodendroglial tumors, that is associated with a better prognosis and 
      enhanced response to therapy. Over the last years, the increasing clinical demand 
      to determine the 1p 19q status has led to the implementation of its testing in 
      many neuropathology laboratories. Several different methods for 1p 19q testing 
      are available: PCR-based loss of heterozygosity analysis, multiplex 
      ligation-dependent probe amplification, array comparative genomic hybridization, 
      and fluorescence in situ hybridization (FISH). Herein, we focus and critically 
      discuss the latter method because a detailed description of procedures and 
      protocols for FISH-based 1p 19q testing in practice is lacking. We present a 
      practical approach to the FISH-based assessment of the 1p 19q status in 
      oligodendroglial tumors, from commonly used locus-specific probes and technical 
      protocols to the neuropathological interpretation of results. Thereby, we aim to 
      facilitate the implementation of FISH-based 1p 19q testing for clinical purposes 
      in standard neuropathology laboratories without special focus on brain tumor 
      research.
FAU - Woehrer, A
AU  - Woehrer A
AD  - Institute of Neurology, CCC-CNS Medical University of Vienna, Waehringer Guertel 
      18-20, 1097 Vienna, Austria.
FAU - Sander, P
AU  - Sander P
FAU - Haberler, C
AU  - Haberler C
FAU - Kern, S
AU  - Kern S
FAU - Maier, H
AU  - Maier H
FAU - Preusser, M
AU  - Preusser M
FAU - Hartmann, C
AU  - Hartmann C
FAU - Kros, J M
AU  - Kros JM
FAU - Hainfellner, J A
AU  - Hainfellner JA
CN  - Research Committee of the European Confederation of Neuropathological Societies
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Clin Neuropathol
JT  - Clinical neuropathology
JID - 8214420
SB  - IM
MH  - Brain Neoplasms/genetics
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Glioma/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
EDAT- 2011/02/19 06:00
MHDA- 2011/06/24 06:00
CRDT- 2011/02/19 06:00
PHST- 2011/02/19 06:00 [entrez]
PHST- 2011/02/19 06:00 [pubmed]
PHST- 2011/06/24 06:00 [medline]
AID - 8414 [pii]
AID - 10.5414/npp30047 [doi]
PST - ppublish
SO  - Clin Neuropathol. 2011 Mar-Apr;30(2):47-55. doi: 10.5414/npp30047.