PMID- 21330950
OWN - NLM
STAT- MEDLINE
DCOM- 20110930
LR  - 20110418
IS  - 1540-0514 (Electronic)
IS  - 1073-2322 (Linking)
VI  - 35
IP  - 5
DP  - 2011 May
TI  - Serum heat shock protein 70 levels, oxidant status, and mortality in sepsis.
PG  - 466-70
LID - 10.1097/SHK.0b013e31820fe704 [doi]
AB  - Animal studies as well as prospective randomized clinical trials associated 
      sepsis with redox imbalance and oxidative stress, but other studies failed to 
      establish a correlation between antioxidant-based therapies and improvement of 
      sepsis condition. This is also true for studies on the role of the chaperone heat 
      shock protein 70 (HSP70), which is increased in serum during sepsis. Heat shock 
      protein 70 is affected at several levels by oxidative stress, but this 
      relationship has never been studied in sepsis. Here, we evaluated the 
      relationship between serum HSP70 immunocontent and oxidant status in sepsis. 
      Patients with severe sepsis were followed up for 28 days after diagnosis, or 
      until death. Up to a maximum of 12 h after sepsis diagnosis, serum was collected 
      for determination of HSP70 immunocontent by Western blot and evaluation of 
      oxidative parameters (TRAP [total radical-trapping antioxidant parameter], TBARSs 
      [thiobarbituric acid-reactive substances], and carbonyl levels). Serum of sepsis 
      patients presented enhanced HSP70 levels. Analysis of oxidative parameters 
      revealed that septic patients with pronounced oxidative damage in serum had also 
      increased HSP70 serum levels. Sepsis patients in whom serum oxidative stress 
      markers were not different from control presented normal serum HSP70. Analysis of 
      septic patients according to survival outcome also indicated that patients with 
      increased HSP70 serum levels presented increased mortality. We concluded that 
      serum HSP70 levels are modulated according to the patient oxidant status, and 
      increased serum HSP70 is associated to mortality in sepsis.
FAU - Gelain, Daniel Pens
AU  - Gelain DP
AD  - Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Instituto de 
      Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto 
      Alegre, Rio Grande do Sul, Brazil. dgelain@yahoo.com.br
FAU - de Bittencourt Pasquali, Matheus Augusto
AU  - de Bittencourt Pasquali MA
FAU - M Comim, Clarissa
AU  - M Comim C
FAU - Grunwald, Marcelo Sartori
AU  - Grunwald MS
FAU - Ritter, Cristiane
AU  - Ritter C
FAU - Tomasi, Cristiane Damiani
AU  - Tomasi CD
FAU - Alves, Sarah Cascaes
AU  - Alves SC
FAU - Quevedo, Joao
AU  - Quevedo J
FAU - Dal-Pizzol, Felipe
AU  - Dal-Pizzol F
FAU - Moreira, Jose Claudio Fonseca
AU  - Moreira JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Shock
JT  - Shock (Augusta, Ga.)
JID - 9421564
RN  - 0 (Antioxidants)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antioxidants/metabolism
MH  - Female
MH  - HSP70 Heat-Shock Proteins/*blood
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Oxidative Stress/*physiology
MH  - Sepsis/*blood
MH  - Thiobarbituric Acid Reactive Substances/metabolism
EDAT- 2011/02/19 06:00
MHDA- 2011/10/01 06:00
CRDT- 2011/02/19 06:00
PHST- 2011/02/19 06:00 [entrez]
PHST- 2011/02/19 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
AID - 10.1097/SHK.0b013e31820fe704 [doi]
PST - ppublish
SO  - Shock. 2011 May;35(5):466-70. doi: 10.1097/SHK.0b013e31820fe704.