PMID- 21333014
OWN - NLM
STAT- MEDLINE
DCOM- 20110503
LR  - 20211020
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 8
DP  - 2011 Feb 19
TI  - Polymorphic genetic characterization of the ORF7 gene of porcine reproductive and 
      respiratory syndrome virus (PRRSV) in China.
PG  - 73
LID - 10.1186/1743-422X-8-73 [doi]
AB  - BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) exhibits 
      extensive genetic variation. The outbreak of a highly pathogenic PRRS in 2006 led 
      us to investigate the extent of PRRSV genetic diversity in China. To this end, we 
      analyzed the Nsp2 and ORF7 gene sequences of 98 Chinese PRRSV isolates. RESULTS: 
      Preliminary analysis indicated that highly pathogenic PRRSV strains with a 
      30-amino acid deletion in the Nsp2 protein are the dominant viruses circulating 
      in China. Further analysis based on ORF7 sequences revealed that all Chinese 
      isolates were divided into 5 subgroups, and that the highly pathogenic PRRSVs 
      were distantly related to the MLV or CH-1R vaccine, raising doubts about the 
      efficacy of these vaccines. The ORF7 sequence data also showed no apparent 
      associations between geographic or temporal origin and heterogeneity of PRRSV in 
      China. CONCLUSION: These findings enhance our knowledge of the genetic 
      characteristics of Chinese PRRSV isolates, and may facilitate the development of 
      effective strategies for monitoring and controlling PRRSV in China.
FAU - Hao, Xiaofang
AU  - Hao X
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, No, 1 Xujiaping, 
      Yanchangbao, Lanzhou, Gansu 730046, PR China.
FAU - Lu, Zengjun
AU  - Lu Z
FAU - Kuang, Wendong
AU  - Kuang W
FAU - Sun, Pu
AU  - Sun P
FAU - Fu, Yu
AU  - Fu Y
FAU - Wu, Lei
AU  - Wu L
FAU - Zhao, Qing
AU  - Zhao Q
FAU - Bao, Huifang
AU  - Bao H
FAU - Fu, Yuanfang
AU  - Fu Y
FAU - Cao, Yimei
AU  - Cao Y
FAU - Li, Pinghua
AU  - Li P
FAU - Bai, Xingwen
AU  - Bai X
FAU - Li, Dong
AU  - Li D
FAU - Liu, Zaixin
AU  - Liu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110219
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - China/epidemiology
MH  - Cluster Analysis
MH  - Genotype
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - *Polymorphism, Genetic
MH  - Porcine Reproductive and Respiratory Syndrome/*epidemiology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Deletion
MH  - Sequence Homology, Amino Acid
MH  - Swine
MH  - Viral Proteins/*genetics
PMC - PMC3049123
EDAT- 2011/02/22 06:00
MHDA- 2011/05/04 06:00
PMCR- 2011/02/19
CRDT- 2011/02/22 06:00
PHST- 2010/12/20 00:00 [received]
PHST- 2011/02/19 00:00 [accepted]
PHST- 2011/02/22 06:00 [entrez]
PHST- 2011/02/22 06:00 [pubmed]
PHST- 2011/05/04 06:00 [medline]
PHST- 2011/02/19 00:00 [pmc-release]
AID - 1743-422X-8-73 [pii]
AID - 10.1186/1743-422X-8-73 [doi]
PST - epublish
SO  - Virol J. 2011 Feb 19;8:73. doi: 10.1186/1743-422X-8-73.