PMID- 21334815
OWN - NLM
STAT- MEDLINE
DCOM- 20111024
LR  - 20131121
IS  - 1879-1409 (Electronic)
IS  - 0305-4179 (Linking)
VI  - 37
IP  - 5
DP  - 2011 Aug
TI  - Designing of mouse model: a new approach for studying sulphur mustard-induced 
      skin lesions.
PG  - 851-64
LID - 10.1016/j.burns.2010.12.010 [doi]
AB  - This study was planned to design a mouse model for studying sulphur mustard 
      (SM)-induced skin injury. SM was applied dermally at dose of 5 or 10 mg kg(-1) in 
      polyethyleneglycol-300 (PEG-300) or dimethylsulphoxide (DMSO) or acetone once. 
      The changes in body weight, organ body weight indices (OBWI) and haematological 
      and oxidative stress parameters were investigated over a period of 3-7 days and 
      supported by histopathological observations. Exposure to SM in PEG-300 or DMSO 
      resulted in a significant depletion in body weight, OBWI, hepatic glutathione 
      (GSH) and elevation in hepatic lipid peroxidation, without affecting the blood 
      GSH and hepatic oxidised glutathione (GSSG) levels. Interestingly, no aforesaid 
      change was observed after dermal application of SM diluted in acetone. These 
      biochemical changes were supported by the histological observations, which 
      revealed pronounced toxic effect and damage to liver, kidney and spleen after 
      dermal application of SM diluted in PEG-300 or DMSO. The skin showed similar 
      microscopic changes after dermal application of SM in all the three diluents, 
      however; the severity of lesions was found to be time and dose dependent. It can 
      be concluded that dermal exposure of SM diluted in acetone can be used to mimic 
      SM-induced skin toxicity without systemic toxicity in a mouse model.
CI  - Copyright (c) 2010 Elsevier Ltd and ISBI. All rights reserved.
FAU - Lomash, Vinay
AU  - Lomash V
AD  - Division of Pharmacology and Toxicology, Defence R & D Establishment, Jhansi 
      Road, Gwalior 474 002, Madhya Pradesh, India.
FAU - Deb, Utsab
AU  - Deb U
FAU - Rai, Renuka
AU  - Rai R
FAU - Jadhav, Sunil E
AU  - Jadhav SE
FAU - Vijayaraghavan, R
AU  - Vijayaraghavan R
FAU - Pant, S C
AU  - Pant SC
LA  - eng
PT  - Journal Article
DEP - 20110222
PL  - Netherlands
TA  - Burns
JT  - Burns : journal of the International Society for Burn Injuries
JID - 8913178
RN  - 0 (Chemical Warfare Agents)
RN  - 1364PS73AF (Acetone)
RN  - GAN16C9B8O (Glutathione)
RN  - T8KEC9FH9P (Mustard Gas)
SB  - IM
MH  - Acetone/pharmacology
MH  - Animals
MH  - Blood Cell Count
MH  - Body Weight/drug effects
MH  - Burns, Chemical/blood/*etiology/pathology
MH  - Chemical Warfare Agents/*toxicity
MH  - Disease Models, Animal
MH  - Glutathione/analysis
MH  - Kidney/pathology
MH  - Lipid Peroxidation/drug effects
MH  - Liver/metabolism/pathology
MH  - Mice
MH  - Mustard Gas/*toxicity
MH  - Skin/*injuries
MH  - Spleen/pathology
EDAT- 2011/02/22 06:00
MHDA- 2011/10/25 06:00
CRDT- 2011/02/22 06:00
PHST- 2010/07/15 00:00 [received]
PHST- 2010/10/20 00:00 [revised]
PHST- 2010/12/10 00:00 [accepted]
PHST- 2011/02/22 06:00 [entrez]
PHST- 2011/02/22 06:00 [pubmed]
PHST- 2011/10/25 06:00 [medline]
AID - S0305-4179(10)00330-X [pii]
AID - 10.1016/j.burns.2010.12.010 [doi]
PST - ppublish
SO  - Burns. 2011 Aug;37(5):851-64. doi: 10.1016/j.burns.2010.12.010. Epub 2011 Feb 22.