PMID- 2133655
OWN - NLM
STAT- MEDLINE
DCOM- 19920402
LR  - 20190918
IS  - 0013-9432 (Print)
IS  - 0013-9432 (Linking)
VI  - 44
IP  - 1-4
DP  - 1990
TI  - Coordinate post-transcriptional regulation of ferritin and transferrin receptor 
      expression: the role of regulated RNA-protein interaction.
PG  - 28-41
AB  - Excess iron results in an increase in the translation of ferritin mRNA and a 
      decrease in the stability of transferrin receptor (TfR) mRNA. These coordinate 
      regulatory events are mediated by similar sequence/structure motifs that exist 
      within the 5' untranslated region (5'UTR) of the ferritin mRNA and the 3'UTR of 
      the TfR mRNA. We have referred to these motifs as iron-responsive elements 
      (IREs). The IREs from both transcripts interact with a cytoplasmic protein that 
      we have called the IRE-binding protein (IRE-BP). The activity but not the amount 
      of the IRE-BP is dependent on the cellular iron status. The biochemical basis for 
      the altered activity of the IRE-BP appears to be the reversible 
      oxidation-reduction of one or more cysteines in the IRE-BP. The IRE-BP is a 90- 
      to 95-kD cytosolic protein that has been purified to homogeneity by RNA affinity 
      chromatography, and the cDNA corresponding to the IRE-BP has been molecularly 
      cloned. Collectively, our data support a model in which the interaction between 
      the IRE-BP and the ferritin IRE results in attenuation of translation, and 
      similar interaction with TfR mRNA can protect the transcript from rapid 
      degradation mediated by a rapid turnover determinant within the 3'UTR.
FAU - Harford, J B
AU  - Harford JB
AD  - Cell Biology and Metabolism Branch, National Institutes of Child Health and Human 
      Development, National Institutes of Health, Bethesda, Md.
FAU - Klausner, R D
AU  - Klausner RD
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Switzerland
TA  - Enzyme
JT  - Enzyme
JID - 1262265
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Transferrin)
RN  - 9007-73-2 (Ferritins)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Base Sequence
MH  - Ferritins/*genetics
MH  - Iron/*metabolism
MH  - Models, Biological
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - *RNA Processing, Post-Transcriptional
MH  - RNA, Messenger/metabolism
MH  - Receptors, Transferrin/*genetics
MH  - Regulatory Sequences, Nucleic Acid
MH  - Sequence Homology, Nucleic Acid
RF  - 45
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1159/000468745 [doi]
PST - ppublish
SO  - Enzyme. 1990;44(1-4):28-41. doi: 10.1159/000468745.