PMID- 21338951
OWN - NLM
STAT- MEDLINE
DCOM- 20110919
LR  - 20220316
IS  - 1673-8527 (Print)
IS  - 1673-8527 (Linking)
VI  - 38
IP  - 1
DP  - 2011 Jan
TI  - Comparison of the distribution of the repetitive DNA sequences in three variants 
      of Cucumis sativus reveals their phylogenetic relationships.
PG  - 39-45
LID - 10.1016/j.jcg.2010.12.005 [doi]
AB  - Repetitive DNA sequences with variability in copy number or/and sequence 
      polymorphism can be employed as useful molecular markers to study phylogenetics 
      and identify species/chromosomes when combined with fluorescence in situ 
      hybridization (FISH). Cucumis sativus has three variants, Cucumis sativus L. var. 
      sativus, Cucumis sativus L. var. hardwickii and Cucumis sativus L. var. 
      xishuangbannesis. The phylogenetics among these three variants has not been well 
      explored using cytological landmarks. Here, we concentrate on the organization 
      and distribution of highly repetitive DNA sequences in cucumbers, with emphasis 
      on the differences between cultivar and wild cucumber. The diversity of 
      chromosomal karyotypes in cucumber and its relatives was detected in our study. 
      Thereby, sequential FISH with three sets of multi-probe cocktails (combined 
      repetitive DNA with chromosome-specific fosmid clones as probes) were conducted 
      on the same metaphase cell, which helped us to simultaneously identify each of 
      the 7 metaphase chromosomes of wild cucumber C. sativus var. hardwickii. A 
      standardized karyotype of somatic metaphase chromosomes was constructed. Our data 
      also indicated that the relationship between cultivar cucumber and C. s. var. 
      xishuangbannesis was closer than that of C. s. var. xishuangbannesis and C. s. 
      var. hardwickii.
CI  - Copyright (c) 2011. Published by Elsevier Ltd.
FAU - Zhao, Xin
AU  - Zhao X
AD  - National Maize Improvement Center of China, Key Laboratory of Crop Genetic 
      Improvement and Genome of Ministry of Agriculture, Beijing Key Laboratory of Crop 
      Genetic Improvement, China Agricultural University, Beijing 100193, China.
FAU - Lu, Jingyuan
AU  - Lu J
FAU - Zhang, Zhonghua
AU  - Zhang Z
FAU - Hu, Jiajin
AU  - Hu J
FAU - Huang, Sanwen
AU  - Huang S
FAU - Jin, Weiwei
AU  - Jin W
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Genet Genomics
JT  - Journal of genetics and genomics = Yi chuan xue bao
JID - 101304616
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Chromosomes, Plant/genetics
MH  - Cucumis sativus/*classification/cytology/*genetics
MH  - DNA, Plant/*genetics
MH  - Genome, Plant/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Metaphase/genetics
MH  - *Phylogeny
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Species Specificity
MH  - Tandem Repeat Sequences/genetics
EDAT- 2011/02/23 06:00
MHDA- 2011/09/20 06:00
CRDT- 2011/02/23 06:00
PHST- 2010/12/01 00:00 [received]
PHST- 2010/12/20 00:00 [revised]
PHST- 2010/12/24 00:00 [accepted]
PHST- 2011/02/23 06:00 [entrez]
PHST- 2011/02/23 06:00 [pubmed]
PHST- 2011/09/20 06:00 [medline]
AID - S1673-8527(10)00018-4 [pii]
AID - 10.1016/j.jcg.2010.12.005 [doi]
PST - ppublish
SO  - J Genet Genomics. 2011 Jan;38(1):39-45. doi: 10.1016/j.jcg.2010.12.005.