PMID- 21340900
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20110222
IS  - 1543-1894 (Print)
IS  - 1543-1894 (Linking)
VI  - 57
DP  - 2001
TI  - Fluorescence itIn Situ Hybridization.
PG  - 199-221
LID - 10.1385/1-59259-136-1:199 [doi]
AB  - In situ hybridization describes the annealing of a labeled nucleic acid to 
      complementary nucleic acid sequences in a fixed target (e.g., chromosomes, free 
      nuclei, nuclei in tissue sections, and DNA) followed by visualisation of the 
      location of the probe. Since its development about 30 yr ago (1,2), it has 
      transformed into a highly effective and rapid technique for uses such as 
      characterizing chromosome aberrations, gene mapping, and marker ordering as well 
      as expression studies.All in situ hybridization originally used radioactively 
      labeled probes, and methodology for in situ hybridization using radioactive 
      probes is covered in Chapter 13 by Poulsom. The strict regulations on 
      radioactivity, long exposure times, and some practical difficulties with the use 
      of radioactive labels limited the wide application of the technique. In the 
      1980s, several methods using non-radioactive labeling were developed (3-7). The 
      ease and effectiveness of fluorescence methods (fluorescence in situ 
      hybridization [FISH]) in particular have now almost rendered the radioisotopic 
      techniques obsolete. FISH has been developed to incorporate chromosome painting 
      and the analysis of the whole genome for aberrations using the approaches of 
      comparative genomic hybridization (CGH; described in detail in Chapter 15 by 
      Roylance), multifluor FISH (M-FISH), and spectral karyotyping (SKY).In FISH, 
      essentially the probes are labeled either directly or indirectly with various 
      fluorochrome dyes such as fluorescein isothiocyanate (FITC) and tetramethyl 
      rhodamine that fluoresce at different wavelengths when excited by ultraviolet 
      (UV) light.
FAU - Goker, H
AU  - Goker H
AD  - Gene Function and Regulation, Institute of Cancer Research, Chester Beatty 
      Laboratories, London, UK.
FAU - Shipley, J
AU  - Shipley J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Med
JT  - Methods in molecular medicine
JID - 101123138
EDAT- 2001/01/01 00:00
MHDA- 2001/01/01 00:01
CRDT- 2011/02/23 06:00
PHST- 2011/02/23 06:00 [entrez]
PHST- 2001/01/01 00:00 [pubmed]
PHST- 2001/01/01 00:01 [medline]
AID - 10.1385/1-59259-136-1:199 [doi]
PST - ppublish
SO  - Methods Mol Med. 2001;57:199-221. doi: 10.1385/1-59259-136-1:199.