PMID- 21340901
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20110222
IS  - 1543-1894 (Print)
IS  - 1543-1894 (Linking)
VI  - 57
DP  - 2001
TI  - Comparative genomic hybridization.
PG  - 223-40
LID - 10.1385/1-59259-136-1:223 [doi]
AB  - Comparative genomic hybridization (CGH) is a molecular cytogenetic technique used 
      to screen the entire genome for gains and losses of genetic material (1). It is 
      being used increasingly in the study of cancer genetics to identify genes 
      important in the initiation, progression, and, of particular relevance here, 
      metastasis of tumors (2-4).One of the advantages of the technique is that the 
      entire genome is examined in a single experiment, so there is no necessity to 
      know the genetic region of interest prior to investigation. Once regions of gain 
      or loss have been identified, these regions can be defined further using 
      fluorescence in situ hybridization (FISH) (described in Chapter 14 by Goker and 
      Shipley) or molecular genetic techniques. CGH is essentially a modified in situ 
      hybridization. Differentially labeled test or tumor DNA (green) and reference or 
      normal DNA (red) are cohybridized to normal metaphase spreads. Differences in the 
      copy number between test and reference DNA are seen as differences in the ratio 
      of green to red fluorescence intensity on the metaphase chromosomes. Images of 
      the metaphases are captured and quantification of the fluorescence ratios 
      performed using a digital image analysis system. Regions of chromosomal gain are 
      seen as an increased fluorescence ratio whereas regions of loss are seen as a 
      decrease in the fluorescence ratio. Losses are detectable when the region 
      affected exceeds 10 Mb (5), smaller regions of gain are detected if there is high 
      level amplification, for example a 2-Mb region that is amplified five times will 
      be visualized (6). For each tumor, 5/210 metaphases are analyzed and an average 
      fluorescence ratio for each chromosome obtained.
FAU - Roylance, R
AU  - Roylance R
AD  - Molecular and Population Genetics Laboratory, Imperial Cancer Research Fund, 
      London, UK.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Med
JT  - Methods in molecular medicine
JID - 101123138
EDAT- 2001/01/01 00:00
MHDA- 2001/01/01 00:01
CRDT- 2011/02/23 06:00
PHST- 2011/02/23 06:00 [entrez]
PHST- 2001/01/01 00:00 [pubmed]
PHST- 2001/01/01 00:01 [medline]
AID - 10.1385/1-59259-136-1:223 [doi]
PST - ppublish
SO  - Methods Mol Med. 2001;57:223-40. doi: 10.1385/1-59259-136-1:223.